Cat. No. 114 003 | 50 μg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 μl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. |
Applications |
WB: 1 : 1000 (AP staining) gallery IP: yes (see remarks) ICC: not recommended IHC: not recommended (see remarks) IHC-P/FFPE: not tested yet ELISA: yes (see remarks) |
Immunogen | Synthetic peptide corresponding to AA 35 to 53 from rat GluN1 (UniProt Id: P35439) |
Reactivity |
Reacts with: human (Q05586), rat (P35439), mouse (P35438). No signal: zebrafish. Other species not tested yet. |
Specificity | Specific for GluN 1. |
matching control protein/peptide | 114-0P |
Remarks |
IP: For most effective IP use the solubilization protocol described in the ELISA protocol. Consider that protein-protein interaction may be affected. IHC: Antibody 2, cat. no. 114 103, is recommended for IHC. ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 114 011 or 114 001 as capture antibody (protocol for sandwich-ELISA). |
Datasheet | 114_003.pdf |