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產(chǎn)品名稱: Gephyrin - 147 008
產(chǎn)品型號: 147 008
產(chǎn)品展商: 其他品牌
產(chǎn)品文檔: 無相關文檔
簡單介紹
Gephyrin - 147 008
Gephyrin - 147 008
的詳細介紹
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Cat. No. 147 008
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50 μg purified IgG, lyophilized. For reconstitution add 50 μl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
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Applications
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WB: yes (see remarks)
IP: yes (see remarks)
ICC: 1 : 500 gallery
IHC: 1 : 500 up to 1 : 1000 (see remarks) gallery
IHC-P/FFPE: not tested yet
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Clone
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RbmAb7a
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Subtype
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IgG1 (κ light chain)
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Immunogen
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Recombinant protein corresponding to AA 1 to 768 from rat Gephyrin (UniProt Id: Q03555)
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Epitop
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Epitop: AA 264 to 276 from rat Gephyrin (UniProt Id: Q03555)
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Reactivity
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Reacts with: human (Q9NQX3), rat (Q03555), mouse (Q8BUV3), pig, goldfish, zebrafish.
Other species not tested yet.
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Specificity
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Specific for the brain specific 93 kDa splice variant. (K.O. verified)
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Remarks
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WB: Clone 3B11 (cat. no. 147 111) highly recommended. IP: Clone 3B11 (cat. no. 147 111) highly recommended. IHC: For best results use the protocol of Schneider Gasser et al., 2006.
Alternatively AGR (10 mM citrate, pH 6.0, over night at 60°C) can be applied.
This antibody is a chimeric antibody based on the well known monoclonal mouse antibody mAb7a. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. The antibody can therefore be used with standard anti-rabbit secondary reagents.
The antibody has been expressed in mammalian cells.
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Datasheet
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147_008.pdf
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