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產(chǎn)品名稱: VAMP 1/2/3 - 104 011
產(chǎn)品型號: 104 011
產(chǎn)品展商: 其他品牌
產(chǎn)品文檔: 無相關文檔
簡單介紹
VAMP 1/2/3 - 104 011
VAMP 1/2/3 - 104 011
的詳細介紹
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Cat. No. 104 011
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100 μg purified IgG, lyophilized. For reconstitution add 100 μl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
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Applications
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WB: 1 : 1000 (AP staining) gallery
IP: yes
ICC: 1 : 100 up to 1 : 500 (see remarks) gallery
IHC: 1 : 100 up to 1 : 500 gallery
IHC-P/FFPE: 1 : 500 up to 1 : 1000 gallery
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Clone
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10.1
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Subtype
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IgG1
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Immunogen
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Recombinant protein corresponding to AA 1 to 118 from rat Synaptobrevin1 (UniProt Id: Q63666)
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Epitop
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Epitop: AA 31 to 98 from rat Synaptobrevin1 (UniProt Id: Q63666)
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Reactivity
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Reacts with: human (P23763, P63027, Q15836), rat (Q63666, P63045, P63025), mouse (Q62442, P63044, P63024), vertebrates, invertebrates.
Other species not tested yet.
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Specificity
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Specific for all three isoforms VAMP 1, 2, 3.
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Remarks
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ICC: ICC/IHC: This antibody is not recommended for non-neuronal cells. Please use cat. no. 104 102 for such experiments.
This antibody reacts with the conserved middle-portion of the molecule. Its affinity/avidity is not very high, thus requiring extended incubation periods and sensitive detection systems.
This antibody recognizes the Botulinumtoxin B cleavage product (aa 1 - 76) with reduced affinity. The sensitivity is sufficient for the detection of cleaved recombinant protein. For analysis of toxin treated tissue homogenates cat. no. 104 203 is recommended.
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Datasheet
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104_011.pdf
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