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產(chǎn)品名稱: VGLUT 1 - 135 311
產(chǎn)品型號: 135 311
產(chǎn)品展商: 其他品牌
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
VGLUT 1 - 135 311
VGLUT 1 - 135 311
的詳細介紹
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Cat. No. 135 311
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100 μg purified IgG, lyophilized. For reconstitution add 100 μl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
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Applications
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WB: 1 : 500 up to 1 : 2000 (AP staining) (see remarks) gallery
IP: yes (see remarks)
ICC: 1 : 100 gallery
IHC: 1 : 100 gallery
IHC-P/FFPE: 1 : 100 up to 1 : 500 gallery
ELISA: yes (see remarks)
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Clone
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317D5
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Subtype
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IgG2a (κ light chain)
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Immunogen
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Recombinant protein corresponding to AA 456 to 560 from rat VGLUT1 (UniProt Id: Q62634)
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Epitop
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Epitop: AA 542 to 560 from rat VGLUT1 (UniProt Id: Q62634)
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Reactivity
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Reacts with: rat (Q62634), mouse (Q3TXX4).
Other species not tested yet.
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Specificity
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Specific for VGLUT 1. (K.O. verified)
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matching control protein/peptide
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135-3P
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Remarks
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WB: This antibody yields stronger signals in Western blot experiments than cat. no. 135 511 but is less sensitive than cat. no. 135 011 and the polyclonal VGluT 1 antibodies. IP: Coupling to protein A is recommended for IP, since covalent coupling to activated sepharose leads to considerable loss of activity. ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 135 303 as detector antibody (protocol for sandwich-ELISA).
VGLUT 1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
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Datasheet
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135_311.pdf
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