Superoxide dismutase [Mn], MnSOD, soda, b3908, JW3879.

Superoxide dismutase Mn (soda) belongs to the iron/manganese superoxide dismutase family. SodA destroys radicals that are typically produced within the cells and which are toxic to biological systems. SodA works by catalyzing the dismutation of the superoxide radical O₂- to O₂ and H₂O₂, which are then metabolized to H₂O and O₂ by catalase and glutathione peroxidase.

SODA produced in E.Coli is a single, non-glycosylated polypeptide chain containing 226 amino acids (1-206 a.a.) and having a molecular mass of 25.2kDa.
SODA is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Escherichia Coli.

Sterile filtered colorless solution.

SODA?E.coli solution (1mg/ml) containining 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 10% glycerol and 0.1M NaCl.

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.

Greater than 95% as determined by SDS-PAGE.

MGSSHHHHHH SSGLVPRGSH MSYTLPSLPY AYDALEPHFD KQTMEIHHTK HHQTYVNNAN AALESLPEFA NLPVEELITK LDQLPADKKT VLRNNAGGHA NHSLFWKGLK KGTTLQGDLK AAIERDFGSV DNFKAEFEKA AASRFGSGWA WLVLKGDKLA VVSTANQDSP LMGEAISGAS
GFPIMGLDVW EHAYYLKFQN RRPDYIKEFW NVVNWDEAAA RFAAKK.

Specific activity is > 350 units/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and xanthine oxidase at pH 7.8 at 25C in a 1.5 ml reaction volume.

ProSpec's products are furnished for?LABORATORY RESEARCH?USE?ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.