Nucleosome core proteins are a group of proteins which are necessary for DNA packaging in eukaryotes. Every core contains an octamer with 2 copies of each histone 2A, 2B, 3 and 4 around which an approx. 140 bp section of DNA is wound twice.
Nucleosome Bovine is purified from bovine tissues by proprietary protein-chemical techniques.
Sterile Filtered clear solution.
Nucleosome Bovine is supplied in 20mM HEPES buffer pH-7.6, 250mM NaCl and 20% glycerol.
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Greater than 80.0% as determined by SDS-PAGE.
0.2-0.5 μg/ml (depending on the type of ELISA plate and coating buffer). Suitable for labeling of functional groups.
1.?Binds IgG type human auto antibodies.
2.?Checkerboard analysis of positive/negative samples.
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.