His tag peptide also called polyhistidine-tag is chain of amino acids that are attached to proteins and consist of at least six histidine (6xHis, Hexa Histidine) residues, often at the N- or C-terminus of the protein. Polyhistidine-tags are used for affinity purification of genetically engineered proteins.
PHISSHG.
Monoclonal antibodies are produced by immunizing mouse with synthesized polypeptide 6 x His, coupled with KLH. This antibody can recognize protein with His-tag in C-terminal.
Mouse IgG1.
Sterile Filtered solution.
1×PBS & 50% glycerol.
Western Blotting: 0.5μg/ml.
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