Catalogue number
ENZ-122
Synonyms
Protein phosphatase 1F, Ca(2+)/calmodulin-dependent protein kinase phosphatase, CaM-kinase phosphatase, CaMKPase, Partner of PIX 2, Protein fem-2 homolog, hFem-2, KIAA0015, POPX2, PPM1F, CAMKP, FEM-2.
Introduction
Protein Phosphatase 1F (PPM1F) is a part of the PP2C family of Ser/Thr protein phosphatases whose members are known to be negative regulators of cell stress response pathways. PPM1F interacts with Rho guanine nucleotide exchange factors (PIX), and as a result blocks the effects of p21-activated kinase 1 (PAK), a protein kinase mediating
biological effects downstream of Rho GTPases. ?Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is one of the substrates of PPM1F. PPM1F is found to be an apoptosis Promoter.
Description
PPM1F Human Recombinant produced in E. coli is a single polypeptide
chain containing 477 amino acids (1-454) and having a molecular mass of
52kDa.
PPM1F is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
E.coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The PPM1F solution (1mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 0.15M NaCl, 20% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMSSGAPQ
KSSPMASGAE ETPGFLDTLL QDFPALLNPE DPLPWKAPGT VLSQEEVEGE LAELAMGFLG
SRKAPPPLAA ALAHEAVSQL LQTDLSEFRK LPREEEEEEE DDDEEEKAPV TLLDAQSLAQ
SFFNRLWEVA GQWQKQVPLA ARASQRQWLV SIHAIRNTRR KMEDRHVSLP SFNQLFGLSD
PVNRAYFAVF DGHGGVDAAR YAAVHVHTNA ARQPELPTDP EGALREAFRR TDQMFLRKAK
RERLQSGTTG VCALIAGATL HVAWLGDSQV ILVQQGQVVK LMEPHRPERQ DEKARIEALG
GFVSHMDCWR VNGTLAVSRA IGDVFQKPYV SGEADAASRA LTGSEDYLLL ACDGFFDVVP
HQEVVGLVQS HLTRQQGSGL RVAEELVAAA RERGSHDNIT VMVVFLRDPQ ELLEGGNQGE
GDPQAEGRRQ DLPSSLPEPE TQAPPRS.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
