ESG1, Developmental pluripotency-associated 5 proteins, hDPPA5, Embryonal stem cell-specific gene 1 protein, ESG-1.

Developmental pluripotency-associated 5 proteins (DPPA5) is a 116 amino acid protein, which localizes to the cytoplasm and contains one KH domain. DPPA5 is expressed in embryonic germ (EG), primordial germ (PG) and embryonic stem (ES) cells. DPPA5 has an imperative role in the maintenance of ES cell pluripotency and may be essential for proper embryogenesis.

DPPA5 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 139 amino acids (1-116 a.a) and having a molecular mass of 15.9kDa.
DPPA5 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Escherichia Coli.

Sterile Filtered clear solution.

DPPA5 protein solution (0.25mg/ml) containing 20mM Phosphate buffer (pH 8.0), and 10% glycerol.

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.

Greater than 85.0% as determined by SDS-PAGE.

MGSSHHHHHH SSGLVPRGSH MGSMGTLPAR RHIPPWVKVP EDLKDPEVFQ VQTRLLKAIF GPDGSRIPYIEQVSKAMLEL KALESSDLTE VVVYGSYLYK LRTKWMLQSM AEWHRQRQER GMLKLAEAMN ALELGPWMK.

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