Tyrosine
3-monooxygenase, Tyrosine 3-hydroxylase, TH.
Tyrosine
3-monooxygenase (Th), is a rate-limiting enzyme in catecholamine synthesis. Th utilizes
tetrahydrobiopterin as well as molecular oxygen to convert tyrosine to
DOPA. Th regulates dopamine (DA)
neurotransmission at the biosynthesis and reuptake steps. Th takes a vital
part in the physiology of adrenergic neurons. Furthermore, Th effects
overexpression in lymphocytes on the differentiation as well as function of T
helper cells.
TH Mouse Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 507 amino acids (1-498a.a.) and having a molecular mass of 57.0kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa).?
TH is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Sf9,
Baculovirus cells.
Sterile Filtered colorless solution.
TH protein solution (0.25mg/ml) containing Phosphate
Buffered Saline (pH 7.4) and 10% glycerol.
Store at 4°C if entire vial will be used within 2-4 weeks.?
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Greater than 90.0% as determined by SDS-PAGE.
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
