PURE - anti mouse TER-119 - Clone TER-119
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產(chǎn)品名稱: PURE - anti mouse TER-119 - Clone TER-119
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PURE - anti mouse TER-119 - Clone TER-119
PURE - anti mouse TER-119 - Clone TER-119
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PURE
- anti Mouse
TER-119 - TER-119
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TER-119
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Rat IgG2b
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Flow Cytometry
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PURE
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0.5 mg/ml
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Mouse Fetal Liver
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Mouse
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The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3)
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RUO
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Liquid
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Store in the dark at 2-8 °C
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TER-119 reacts in ***** mice with mature erythrocytes, 20±25% of bone marrow cells and 2±3% of spleen cells but not with thymocytes nor lymph node cells. In fetal haematopoietic tissues, 30±40% of d 10 yolk sac cells, 80±90% of d 14 fetal liver cells and 40±50% of newborn liver cells were reactive with TER-119. TER-1191 cells in ***** bone marrow expressed significant levels of CD45 but not myeloid (Mac-1, Gr-1) or B-cell (B220) markers. Morphological examination and haematopoietic colony-forming assays for isolated TER- 1191 cells revealed that TER-119 reacts with erythroid cells at differentiation stages from early proerythroblast to mature erythrocyte, but not with cells showing typical erythroid blast-forming unit (BFU-E) and erythroid colonyforming unit (CFU-E) activities. Erythroleukaemia cell lines do not express the TER-119 antigen even after stimulation with dimethylsulphoxide.
TER-119 immunoprecipitated protein bands with molecular masses of 110 kDa, 60 kDa, 52 kDa and 32 kDa from erythrocyte membrane, whereas only a 52-kDa band was detected by TER-119 in Western blot analysis. Further molecular and cellular analyses indicated that the TER-119 antigen is a molecule associated with cell-surface glycophorin A but not with glycophorin A itself.
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Ly-76
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52 kD
