Bromodeoxyuridine (BrdU) Antibody
如果您對(duì)該產(chǎn)品感興趣的話,可以
產(chǎn)品名稱: Bromodeoxyuridine (BrdU) Antibody
產(chǎn)品型號(hào): abx000064
產(chǎn)品展商: 其他品牌
產(chǎn)品文檔: 無相關(guān)文檔
簡(jiǎn)單介紹
Bromodeoxyuridine (BrdU) Antibody
的詳細(xì)介紹
BrdU Antibody is a Mouse Monoclonal antibody against BrdU. The halogenated pyrimidine thymidine analog bromodeoxyuridine (BrdU) is incorporated into newly synthesized DNA strands of S phase cells and is useful for estimating the fraction of cells in S phase. Additionally, the analysis of the uptake of BrdU is a reliable method to quantitate the degree of DNA synthesis. BrdU is also useful for studying sister chromatid exchange and to isolate nascent DNA. UV-induced excision-repair synthesis is one method for incorporating BrdU into cellular DNA. Anti-BrdU antibodies bind to the exposed BrdU in single-stranded DNA after a hydrochloric acid denaturation step or nuclease digestion. Protease antigen recovery is necessary for most tissues or cells fixed with cross-linking agents such as formalin but may decrease the specificity of BrdU immunodetection. The monoclonal antibody Bu20a against BrdU stains BrdU incorporated into the nulcei of a wide range of proliferating cell types including human tumors growing in nude mice and tonsil lymphoid.
| Target |
BrdU |
| Reactivity |
Mouse |
| Host |
Mouse |
| Clonality |
Monoclonal |
| Tested Applications |
IHC, IF/ICC |
| Recommended dilutions |
IHC: 1/50 - 1/500, IF/ICC: 1/50 - 1/500, IF/ICC: 1/50 - 1/500. Optimal dilutions/concentrations should be determined by the end user. |
| Immunogen |
Synthetic Compound. Bromodeoxyuridine. |
| Purification |
Affinity purified. |
| Form |
Liquid |
| Isotype |
IgG |
| Conjugation |
Unconjugated |
| Storage |
Aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Gene Symbol |
BrdU |
| Concentration |
> 1 mg/ml |
| Buffer |
PBS, pH 7.3, 0.02% sodium azide, 50% glycerol. |
| Availability |
Shipped within 5-10 working days. |
| Note |
This product is for research use only. |
