Yamanaka Factor Polycistronic Adenovirus (Human)
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產(chǎn)品名稱: Yamanaka Factor Polycistronic Adenovirus (Human)
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Yamanaka Factor Polycistronic Adenovirus (Human)
Yamanaka Factor Polycistronic Adenovirus (Human)
的詳細(xì)介紹
|
Print Version |
| Vector |
pAdeno |
| Promoter |
CMV |
| Insert |
Yamanaka Factor Polycistronic |
| Picture |
 |
| Organism |
Human |
| Accession # |
Refer to individual adenovirus. |
| Alias |
Oct4, Sox2, KLF4 and Myc |
| Functions |
Yamanaka iPSC factors (Oct4, Sox2, KLF4 and Myc) expressed from a single CMV promoter in a single adenoviral vector. All four factors are linked by protein 2A sequence which allows similar levels of transgene expression of four factors in target cells, thus streamlining iPSC reprogramming. A GFP control adenovirus is included for easy tracking of gene transduction efficiency. |
| Gene Type |
cDNA |
| Purification |
By Ion-Exchange column. |
| Quality Control |
By sequencing and Western blot analysis of transgene expression in transduced cells. Functional test includes iPSC reprogramming. |
| Titer |
>1x106pfu/mL |
| Storage |
10mM Tris-HCl, pH 8.0. 2.5% glycerol |
| Shipping |
High titer adenoviruses are shipped with dry ice and low titer adenoviruses are shipped with ice packs. For long term storage, it is recommended to store the viruses at -80°C in small aliquots to avoid repeated freeze-thaw cycles. |
| Notes |
For Research Use Only, not for therapeutic or diagnostic purposes. |
| References |
Induced pluripotent stem cell lines derived from human somatic cells. Yu et al. Science. 2007 Dec 21;318(5858):1917-20. Epub 2007 Nov 20.
Human induced pluripotent stem cells free of vector and transgene sequences. Yu et al. Science. 2009 May 8;324(5928):797-801. Epub 2009 Mar 26.
Virus-free induction of pluripotency and subsequent excision of reprogramming factors. Kaji et al. Nature. 2009 Apr 9;458(7239):771-5. Epub 2009 Mar 1. |
