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CHOPWT9 iPSC

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產(chǎn)品名稱: CHOPWT9 iPSC
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CHOPWT9 iPSC


CHOPWT9 iPSC  的詳細介紹
Print Version
Safety BioSafety Level II
Organism Human
Donor Female
Source Organ Peripheral Blood Mononuclear Cells
Growth Properties Adherent
Morphology Polygonal
Markers Oct4, Sox2, Nanog, Rex1, DMNT3B, and ABCG2
Picture
Description The CHOPWT9 IPSCs were established by Sendai viral infection carrying reprogramming factors Oct3/4, Sox2, c-Myc, and Klf-4 of peripheral blood mononuclear cells collected from a healthy ***** female. These cells are capable of in vivo tetratoma formation as tested on NOD/SCID mice. The CHOPWT9 IPSCs are valuable in the field of developmental biology as they are useful as a control for applications such as differentiation analyses to the three germ layers and derivative tissues.
Quality Control 1) Flow cytometry analysis of pluripotency markers;
2) Karyotype analysis of chromosomal G-band;
3) qPCR of pluripotency genes
Concentration 106cells/vial
Propagation The iPSCs requires a co-culture with mouse embryonic fibroblasts (MEFs) to proliferate. The base medium for this cell line is Prigrow IV medium available from abm (TM004). To make the completed growth medium, add the following components to the base medium: KnockOutTM Serum Replacement (Invitrogen; A3181502) to final concentration of 15%, 1% NEAA (Invitrogen; 11140050), 1% L-glutamine (G275), 0.1 mM 2-mercaptoethanol (Invitrogen; 21103-049) and 1% Penicillin/Streptomycin Solution (G255). Filter medium with 0.45 μM, before adding 5-10 ng/ml human bFGF (Z101455).Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C.

Add 10 μM ROCK inhibitor (R&D Systems; 1254/50) to media when plating iPSCs on MEFs.
Storage -180°C
Shipping On Dry Ice
Notes For Research Use Only, not for therapeutic or diagnostic purposes.
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