Product Name
FAM126A, siRNA
Full Product Name
FAM126A siRNA (Human)
Product Synonym Names
DRCTNNB1A; Hyccin; Down-regulated by CTNNB1 protein A; Protein FAM126A
Product Gene Name
FAM126A sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9BYI3
Specificity
FAM126A siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human FAM126A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of FAM126A sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
FAM126A sirna
siRNA to inhibit FAM126A expression using RNA interference
Applications Tested/Suitable for FAM126A sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for FAM126A. It may not necessarily be applicable to this product.
NCBI Accession #
NP_115970.2
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NCBI GenBank Nucleotide #
NM_032581.3
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UniProt Primary Accession #
Q9BYI3
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UniProt Secondary Accession #
Q6N010; Q75MR4; Q7LDZ4; Q96MX1; Q96NQ6; A4D145[Other Products]
UniProt Related Accession #
Q9BYI3[Other Products]
Molecular Weight
19,260 Da
NCBI Official Full Name
hyccin
NCBI Official Synonym Full Names
family with sequence similarity 126, member A
NCBI Official Symbol
FAM126A??[Similar Products]
NCBI Official Synonym Symbols
HCC; HLD5; HYCC1; DRCTNNB1A
??[Similar Products]
NCBI Protein Information
hyccin
UniProt Protein Name
Hyccin
UniProt Synonym Protein Names
Down-regulated by CTNNB1 protein A; Protein FAM126A
UniProt Gene Name
FAM126A??[Similar Products]
UniProt Synonym Gene Names
DRCTNNB1A??[Similar Products]
UniProt Entry Name
HYCCI_HUMAN
NCBI Summary for FAM126A
The protein encoded by this gene may play a part in the beta-catenin/Lef signaling pathway. Expression of this gene is down-regulated by beta-catenin. Defects in this gene are a cause of hypomyelination with congenital cataract (HCC). [provided by RefSeq, Oct 2008]
UniProt Comments for FAM126A
FAM126A: May have a role in the beta-catenin/Lef signaling pathway. May have a role in the process of myelination of the central and peripheral nervous system. Defects in FAM126A are the cause of leukodystrophy hypomyelinating type 5 (HLD5). This disorder is characterized by congenital cataract, progressive neurologic impairment, and diffuse myelin deficiency. Affected individuals experience progressive pyramidal and cerebellar dysfunction, muscle weakness and wasting prevailingly in the lower limbs. Mental deficiency ranges from mild to moderate. Belongs to the FAM126 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 7p15.3
Cellular Component: cytoplasm; plasma membrane
Molecular Function: signal transducer activity
Biological Process: signal transduction
Disease: Leukodystrophy, Hypomyelinating, 5
Research Articles on FAM126A
1. A disease-associated FAM126A missense mutation causes protein accumulation in subcellular compartments.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
