Product Name
PITX3, siRNA
Full Product Name
PITX3 siRNA (Rat)
Product Synonym Names
PTX3; Pituitary homeobox 3; Homeobox protein PITX3; Paired-like homeodomain transcription factor 3
Product Gene Name
PITX3 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P81062
Specificity
PITX3 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat PITX3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of PITX3 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
PITX3 sirna
siRNA to inhibit PITX3 expression using RNA interference
Applications Tested/Suitable for PITX3 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for PITX3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_062120.1
[Other Products]
NCBI GenBank Nucleotide #
NM_019247.1
[Other Products]
UniProt Primary Accession #
P81062
[Other Products]
UniProt Related Accession #
P81062[Other Products]
Molecular Weight
31,729 Da
NCBI Official Full Name
pituitary homeobox 3
NCBI Official Synonym Full Names
paired-like homeodomain 3
NCBI Official Symbol
Pitx3??[Similar Products]
NCBI Protein Information
pituitary homeobox 3
UniProt Protein Name
Pituitary homeobox 3
UniProt Synonym Protein Names
Homeobox protein PITX3; Paired-like homeodomain transcription factor 3
Protein Family
Pituitary homeobox
UniProt Gene Name
Pitx3??[Similar Products]
UniProt Synonym Gene Names
Ptx3??[Similar Products]
UniProt Entry Name
PITX3_RAT
NCBI Summary for PITX3
acts as a transcriptional activator; may regulate development of the mesencephalic dopaminergic (mesDA) neuronal lineage [RGD, Feb 2006]
UniProt Comments for PITX3
PITX3: Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. In addition to its importance during development, it also has roles in the long-term survival and maintenance of the mdDA neurons. Activates NR4A2/NURR1- mediated transcription of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons. Acts by decreasing the interaction of NR4A2/NURR1 with the corepressor NCOR2/SMRT which acts through histone deacetylases (HDACs) to keep promoters of NR4A2/NURR1 target genes in a repressed deacetylated state. Essential for the normal lens development and differentiation. Plays a critical role in the maintenance of mitotic activity of lens epithelial cells, fiber cell differentiation and in the control of the temporal and spatial activation of fiber cell-specific crystallins. Positively regulates FOXE3 expression and negatively regulates PROX1 in the anterior lens epithelium, preventing activation of CDKN1B/P27Kip1 and CDKN1C/P57Kip2 and thus maintains lens epithelial cells in cell cycle. Defects in PITX3 are a cause of cataract autosomal dominant (ADC). Cataract is an opacification of the crystalline lens of the eye that frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function. Cataract is the most common treatable cause of visual disability in childhood. Defects in PITX3 are a cause of anterior segment mesenchymal dysgenesis (ASMD); also known as anterior segment ocular dysgenesis (ASOD). ASMD consists of a range of developmental defects in structures at the front of the eye, resulting from abnormal migration or differentiation of the neural crest derived mesenchymal cells that give rise to the cornea, iris, and other components of the anterior chamber during eye development. Mature anterior segment anomalies are associated with an increased risk of glaucoma and corneal opacity. Conditions falling within the phenotypic spectrum include aniridia, posterior embryotoxon, Axenfeld anomaly, Reiger anomaly/syndrome, Peters anomaly, and iridogoniodysgenesis. Defects in PITX3 are the cause of cataract posterior polar type 4 (CTPP4). A subcapsular opacity, usually disk-shaped, located at the back of the lens. It can have a marked effect on visual acuity. Some patients affected by cataract posterior polar type 4 can present a severe phenotype including microphthalmia and neurological dysfunction. Belongs to the paired homeobox family. Bicoid subfamily.
Protein type: DNA-binding; Transcription factor; Cell development/differentiation
Cellular Component: nucleus
Biological Process: lens development in camera-type eye; transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; transcription, DNA-dependent; regulation of gene expression; positive regulation of transcription, DNA-dependent; midbrain development; neuron development; locomotory behavior; lens morphogenesis in camera-type eye; positive regulation of transcription from RNA polymerase II promoter
Research Articles on PITX3
1. Thus, this study demonstrates for the first time a transcription regulatory role for FoxP1 on the Pitx3 gene in mammalian stem cells.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
