ARP1; COUP-TFII; COUPTFB; MGC117452; SVP40; TFCOUP2; ADP-ribosylation factor related protein 1|ovalbumin upstream promoter transcription factor II|transcription factor COUP 2 (chicken ovalbumin upst

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Mouse NR2F2. No significant cross-reactivity or interference between Mouse NR2F2 and analogues was observed.

Store at 2-8 degree C.

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Small volumes of NR2F2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS285810 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the COUP transcription factor 2 (NR2F2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NR2F2. The ELISA analytical biochemical technique of the MBS285810 kit is based on NR2F2 antibody-NR2F2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NR2F2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NR2F2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate NR2F2 in samples. An antibody specific for NR2F2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNR2F2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NR2F2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NR2F2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

45,571 Da

nuclear receptor subfamily 2, group F, member 2

COUP transcription factor 2

COUP transcription factor 2

Aporp1; Arp-1; Arp1; Tfcoup2; COUP-TF2; ARP-1; COUP-TF II??[Similar Products]

NR2F2: Ligand-activated transcription factor. Activated by high concentrations of 9-cis-retinoic acid and all-trans-retinoic acid, but not by dexamethasone, cortisol or progesterone (in vitro). Regulation of the apolipoprotein A-I gene transcription. Binds to DNA site A. Interacts with SQSTM1. Binds DNA as a dimer; homodimer or heterodimer with NR2F6. Interacts with NCOA1, NCOA2, NCOA3 and PPARGC1A. Interacts with ZFPM2. Ubiquitous. Belongs to the nuclear hormone receptor family. NR2 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Motility/polarity/chemotaxis; Nuclear receptor

Chromosomal Location of Human Ortholog: 7 C|7 38.66 cM

Cellular Component: nucleus

Molecular Function: DNA binding; DNA binding transcription factor activity; ligand-dependent nuclear receptor activity; metal ion binding; protein homodimerization activity; retinoic acid binding; sequence-specific DNA binding; steroid hormone receptor activity; zinc ion binding

Biological Process: anterior/posterior pattern specification; blood vessel morphogenesis; fertilization; forebrain development; in utero embryonic development; limb development; lymph vessel development; maternal placenta development; negative regulation of cyclin-dependent protein serine/threonine kinase activity; negative regulation of endothelial cell proliferation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; neuron migration; positive regulation of systemic arterial blood pressure; positive regulation of transcription, DNA-templated; radial pattern formation; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-templated; skeletal muscle tissue development; steroid hormone mediated signaling; transcription, DNA-dependent

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