Product Name
14-3-3 sigma/Stratifin (SFN), Antibody
Full Product Name
Goat anti-14-3-3 sigma/Stratifin, Biotinylated Antibody
Product Synonym Names
Goat Anti-14-3-3 sigma/Stratifin Antibody; SFN; stratifin; YWHAS; 14-3-3 sigma; epithelial cell marker protein 1
Product Gene Name
anti-SFN antibody
[Similar Products]
Matching Pairs
Unconjugated Antibody: 14-3-3 sigma/Stratifin (MBS421444)
Biotin Conjugated Antibody: 14-3-3 sigma/Stratifin (MBS423714)
Antibody/Peptide Pairs
14-3-3 sigma / Stratifin peptide (MBS426009) is used for blocking the activity of 14-3-3 sigma/Stratifin antibody (MBS423714)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P31947
Species Reactivity
Human, Mouse
Expected from sequence similarity: Rat, Dog, Pig
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Immunogen Sequence
Peptide with sequence CDLHTLSEDSYKDST., from the internal region of the protein sequence according to NP_006133.1.
Peptide Sequence + C
CDLHTLSEDSYKDST.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-SFN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-SFN antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-SFN antibody
Pep-ELISA: antibody detection limit dilution 1:16000.
WB: Approx 28kDa band observed in Mouse Skin lysates. Recommended concentration: 0.1-0.3ug/ml.
Immunohistochemistry (IHC) of anti-SFN antibody
Biotinylated (0.1ug/ml) staining of Mouse Skin lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.
NCBI/Uniprot data below describe general gene information for SFN. It may not necessarily be applicable to this product.
NCBI Accession #
NP_006133.1
[Other Products]
NCBI Related Accession #
Manufactured in an ISO 9001:2008 Certified Laboratory.NP_061224.2[Other Products]
NCBI GenBank Nucleotide #
NM_006142.4
[Other Products]
UniProt Primary Accession #
P31947
[Other Products]
UniProt Secondary Accession #
Q6FH30; Q6FH51; Q96DH0[Other Products]
UniProt Related Accession #
P31947[Other Products]
NCBI Official Full Name
14-3-3 protein sigma
NCBI Official Synonym Full Names
stratifin
NCBI Official Symbol
SFN??[Similar Products]
NCBI Official Synonym Symbols
YWHAS
??[Similar Products]
NCBI Protein Information
14-3-3 protein sigma
UniProt Protein Name
14-3-3 protein sigma
UniProt Synonym Protein Names
Epithelial cell marker protein 1; Stratifin
Protein Family
14-3-3 protein
UniProt Gene Name
SFN??[Similar Products]
UniProt Synonym Gene Names
HME1??[Similar Products]
NCBI Summary for SFN
This gene encodes a cell cycle checkpoint protein. The encoded protein binds to translation and initiation factors and functions as a regulator of mitotic translation. In response to DNA damage this protein plays a role in preventing DNA errors during mitosis. [provided by RefSeq, Aug 2017]
UniProt Comments for SFN
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
Research Articles on SFN
1. Review/Meta-analysis: 14-3-3 sigma promoter methylation may be associated with the carcinogenesis of breast cancer and might represent a useful blood-based biomarker for the clinical diagnosis of breast cancer.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
