Anaphase-promoting complex subunit 2; APC2; Cyclosome subunit 2; ANAPC2; APC2; KIAA1406

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This ANAPC2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 712-746 amino acids from the C-terminal region of human ANAPC2.

Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.5 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-ANAPC2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 drives presynaptic differentiation.

Crown Antibodies; Cell Biology; Developmental Biology; Neuroscience; Signal Transduction; Stem Cells

ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)

IHC~~1:25
WB~~1:1000

Immunohistochemical analysis of paraffin-embedded H. thymus section using ANAPC2 Antibody (C-term). MBS9200930 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Western blot analysis of lysate from human testis tissue lysate, using ANAPC2 Antibody (C-term). MBS9200930 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

93828

anaphase promoting complex subunit 2

anaphase-promoting complex subunit 2

Anaphase-promoting complex subunit 2

APC2; KIAA1406; APC2??[Similar Products]

ANC2_HUMAN

A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The product of this gene is a component of the complex and shares sequence similarity with a recently identified family of proteins called cullins, which may also be involved in ubiquitin-mediated degradation. [provided by RefSeq, Jul 2008]

APC2: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Belongs to the cullin family. The APC/C is composed of at least 12 subunits. Interacts through the cullin domain with ANAPC11 and with UBCH10. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: nucleoplasm; anaphase-promoting complex; cytosol

Molecular Function: ubiquitin protein ligase binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; positive regulation of axon extension; mitotic cell cycle spindle assembly checkpoint; positive regulation of synaptic plasticity; positive regulation of dendrite morphogenesis; mitotic cell cycle

Yu H.,et al.Science 279:1219-1222(1998).
Humphray S.J.,et al.Nature 429:369-374(2004).
Nagase T.,et al.DNA Res. 7:65-73(2000).
Nakajima D.,et al.DNA Res. 9:99-106(2002).
Tang Z.,et al.Mol. Biol. Cell 12:3839-3851(2001).

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