Glucose Transporter 2 Antibody; Glucose Transporter GLUT2 Antibody; BLUT-2 Antibody; GTR_Human Antibody; SLC2a2 Antibody; Solute Carrier Family 2 (facilitated glucose transporter) member 2 Antibody; GLUT2 Antibody: PerCP; Rabbit Anti-Human GLUT2 Polyclonal; GLUT2 Antibody

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Peptide Affinity Purified

1 mg/ml (lot specific)

Store at -20 degree C

Small volumes of anti-GLUT2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Glucose transporter 2 (GLUT2) also known as solute carrier family 2 (facilitated glucose transporter), member 2 (SLC2A2) is a transmembrane carrier protein that enables protein facilitated glucose movement across cell membranes. It is the principal transporter for transfer of glucose between liver and blood, and has a role in renal glucose reabsorption (1). Mutations in SLC2A2 lead to Fanconi-Bickel syndrome (FBS), which results in hepatorenal glycogen accumulation, proximal renal tubular dysfunction, and impaired utilization of glucose and galactose. Recent studies have shown that mutations in SLC2A2 can cause neonatal diabetes, and therefore may contribute to human insulin secretion (2). Novel SLC2A2 mutations have also been discovered and are being investigated to determine their roles in FBS as well (3).

Cancer; Cell Signaling; Trafficking

Western Blot (WB)

Western Blot: 1:1000

Western blot analysis of Rat Liver showing detection of ~57.5kDa GLUT2 protein using Rabbit Anti-GLUT2 Polyclonal Antibody (SPC-697). Lane 1: MW Ladder. Lane 2: Rat Liver (20 ug). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-GLUT2 Polyclonal Antibody (SPC-697) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~57.5kDa. Other Band(s): ~37kDa.

Western blot analysis of Human HeLa and HEK293T cell lysates showing detection of ~57.5kDa GLUT2 protein using Rabbit Anti-GLUT2 Polyclonal Antibody (SPC-697). Lane 1: MW Ladder. Lane 2: Human HeLa (20 ug). Lane 3: Human 293T (20 ug). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-GLUT2 Polyclonal Antibody (SPC-697) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~57.5kDa. Other Band(s): ~72kDa.

Predicted molecular weight at ~57.5kDa. Observed molecular weight at 60-70kDa and 38-45kDa.

solute carrier family 2 member 2

solute carrier family 2, facilitated glucose transporter member 2

Solute carrier family 2, facilitated glucose transporter member 2

GLUT2; GLUT-2??[Similar Products]

GTR2_HUMAN

This gene encodes an integral plasma membrane glycoprotein of the liver, islet beta cells, intestine, and kidney epithelium. The encoded protein mediates facilitated bidirectional glucose transport. Because of its low affinity for glucose, it has been suggested as a glucose sensor. Mutations in this gene are associated with susceptibility to diseases, including Fanconi-Bickel syndrome and noninsulin-dependent diabetes mellitus (NIDDM). Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]

GLUT2: an integral membrane facilitative glucose transporter. One of 13 members of the human equilibrative glucose transport protein family. Present at high levels in the plasma membrane of pancreatic b-cells where it initiates the first step in glucose-stimulated insulin secretion, a glucose metabolism-dependent signaling mechanism. May mediate the bidirectional transfer of glucose across the plasma membrane of hepatocytes. May also participate with the Na( +2)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney. Required for glucose sensitivity in the hypothalamus and brain stem. Involved in the control of food intake and stimulation of glucose uptake by peripheral tissues.

Protein type: Membrane protein, integral; Transporter, SLC family; Transporter; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 3q26.1-q26.2

Cellular Component: basolateral plasma membrane; brush border membrane; cytosol; endosome; integral to plasma membrane; intercellular junction; membrane; plasma membrane

Molecular Function: D-glucose transmembrane transporter activity; dehydroascorbic acid transporter activity; glucose transmembrane transporter activity; hexose transmembrane transporter activity; insulin receptor binding

Biological Process: carbohydrate metabolic process; carbohydrate utilization; endocrine pancreas development; energy reserve metabolic process; fructose transport; glucose transport; hexose transport; regulation of insulin secretion; response to glucose stimulus; response to peptide hormone stimulus; transmembrane transport

Disease: Diabetes Mellitus, Noninsulin-dependent; Fanconi-bickel Syndrome

1. Kellett G.L., Brot-Laroche E. (2005) Diabetes. 54(10): 3056-3062. 2. Sansbury F.H., et al. (2012) Diabetologia. 55(9): 2381-2385. 3. Su Z., et al. (2011) J Perdiatr Endocrinol Metab. 24(9-10): 749-753.

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