Product Name
Ribosomal RNA small subunit methyltransferase NEP1 (Emg1), Recombinant Protein
Full Product Name
Recombinant Mouse Ribosomal RNA small subunit methyltransferase NEP1 (Emg1)
Product Gene Name
Emg1 recombinant protein
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
2-244aa, Full Length of Mature Protein
Sequence
SAASGGFQPR ERRFSVQEQD WETTPPKKLR LGAGSKCGGR RLIVVLEGAS LETVKVGKTY ELLNCDRHKS MLLKNGRDPG EVRPDITHQS LLMLMDSPLN RAGLLQVYIH TQKNVLIEVN PQTRIPRTFD RFCGLMVQLL HKLSVRAADG PQKLLKVIKN PVSDHFPVGC MKIGTSFSVE DISDIRELVP SSDPVVFVVG AFAHGKVSVE YTEKMVSISN YPLSAALTCA KVTTAFEEVW GVI
3D Structure
ModBase 3D Structure for O35130
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
>85% (SDS-PAGE) (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Storage Buffer
Tris-based buffer, 50% glycerol
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of Emg1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
NCBI/Uniprot data below describe general gene information for Emg1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_038564.1
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NCBI GenBank Nucleotide #
NM_013536.2
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UniProt Primary Accession #
O35130
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UniProt Related Accession #
O35130[Other Products]
Molecular Weight
26,974 Da
NCBI Official Full Name
ribosomal RNA small subunit methyltransferase NEP1
NCBI Official Synonym Full Names
EMG1 N1-specific pseudouridine methyltransferase
NCBI Official Symbol
Emg1??[Similar Products]
NCBI Official Synonym Symbols
C2f; Grcc2f
??[Similar Products]
NCBI Protein Information
ribosomal RNA small subunit methyltransferase NEP1
UniProt Protein Name
Ribosomal RNA small subunit methyltransferase NEP1
UniProt Synonym Protein Names
18S rRNA (pseudouridine(1248)-N1)-methyltransferase
UniProt Gene Name
Emg1??[Similar Products]
UniProt Comments for Emg1
S-adenosyl-L-methionine-dependent pseudouridine N1-methyltransferase that methylates pseudouridine at position 1248 (Psi1248) in 18S rRNA. Involved the biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) conserved in eukaryotic 18S rRNA. Is not able to methylate uridine at this position. Has also an essential role in 40S ribosomal subunit biogenesis independent on its methyltransferase activity, facilitating the incorporation of ribosomal protein S19 during the formation of pre-ribosomes.
Research Articles on Emg1
1. Data suggest that Essential for Mitotic Growth 1 (EMG1) deficiency caused mitotic arrest and a halt in cell growth.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
