Mouse Ribosomal RNA small subunit methyltransferase NEP1 (EMG1) ELISA kit; BWCNS; C2F; FLJ60792; Grcc2f; NEP1; ribosome biogenesis protein NEP1; EMG1 nucleolar protein homolog (S. cerevisiae)

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C

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Small volumes of EMG1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9332472 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the EMG1 nucleolar protein homolog (S. cerevisiae) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing EMG1. The ELISA analytical biochemical technique of the MBS9332472 kit is based on EMG1 antibody-EMG1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EMG1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EMG1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

26,974 Da

EMG1 nucleolar protein homolog (S. cerevisiae)

ribosomal RNA small subunit methyltransferase NEP1; protein C2f; gene rich cluster, C2f; nucleolar protein EMG1 homolog; ribosome biogenesis protein NEP1; probable ribosome biogenesis protein NEP1; 18S rRNA (pseudouridine-N1-)-methyltransferase NEP1

Ribosomal RNA small subunit methyltransferase NEP1

C2f; Grcc2f??[Similar Products]

NEP1_MOUSE

C2F: Involved in 40S ribosomal subunit biogenesis and 18S rRNA processing. Specifically catalyzes the N1-methylation of pseudouridine at position 1248 (Psi1248) in 18S rRNA. Thus, appears to be the methyltransferase involved in the biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) in position 1248 in 18S rRNA. Is not able to methylate uridine at this position. Defects in EMG1 are the cause of Bowen-Conradi syndrome (BWCNS). BWCNS is a combination of malformations characterized in newborns by low birth weight, microcephaly, mild joint restriction, a prominent nose, micrognathia, fifth finger clinodactyly, and 'rocker-bottom' feet. The syndrome is transmitted as an autosomal recessive trait. The prognosis is poor, with all infants dying within the first few months of life. Belongs to the NEP1 family.

Protein type: Nucleolus; EC 2.1.1.-; RNA-binding

Cellular Component: small subunit processome; nucleolus; nucleus

Molecular Function: methyltransferase activity; monomethylamine methyltransferase activity; trimethylamine methyltransferase activity; RNA binding; tRNA (cytosine)-methyltransferase activity; RNA methyltransferase activity; rRNA (cytosine-C5-967)-methyltransferase activity; rRNA (uridine) methyltransferase activity; N-methyltransferase activity; tRNA (guanine) methyltransferase activity; rRNA (adenine-N6,N6-)-dimethyltransferase activity; arginine N-methyltransferase activity; selenocysteine methyltransferase activity; methanol-specific methylcobalamin:coenzyme M methyltransferase activity; protein-arginine N-methyltransferase activity; tRNA (guanine-N2-)-methyltransferase activity; dimethylamine methyltransferase activity; protein methyltransferase activity; 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity; transferase activity; hydroxyneurosporene-O-methyltransferase activity; rRNA (uridine-2'-O-)-methyltransferase activity; tRNA (uracil) methyltransferase activity; rRNA (adenine) methyltransferase activity; C-methyltransferase activity; cobalt-precorrin-6B C5-methyltransferase activity; cobalt-precorrin-3 C17-methyltransferase activity; 1-phenanthrol methyltransferase activity; S-methyltransferase activity; lysine N-methyltransferase activity; DNA-methyltransferase activity; tRNA (cytosine-5-)-methyltransferase activity; C-terminal protein carboxyl methyltransferase activity; tRNA (adenine)-methyltransferase activity; rRNA (cytosine) methyltransferase activity; cobalt-precorrin-7 C15-methyltransferase activity; protein-arginine N5-methyltransferase activity; rRNA methyltransferase activity; protein-leucine O-methyltransferase activity; demethylmenaquinone methyltransferase activity; rRNA (adenine-N6-)-methyltransferase activity; tRNA methyltransferase activity; rRNA (guanine) methyltransferase activity; methylarsonite methyltransferase activity; rRNA binding; mRNA methyltransferase activity; methylamine-specific methylcobalamin:coenzyme M methyltransferase activity; tRNA (guanine-N1-)-methyltransferase activity; cobalt-precorrin-5B C1-methyltransferase activity; protein-lysine N-methyltransferase activity; O-methyltransferase activity; tRNA (adenine-57, 58-N(1)-) methyltransferase activity; tRNA (adenine-N1-)-methyltransferase activity

Biological Process: methylation; maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); blastocyst development; nucleologenesis; rRNA methylation; ribosome biogenesis and assembly; rRNA processing

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