Product Name
EMG1, Blocking Peptide
Full Product Name
EMG1 Peptide
Product Gene Name
EMG1 blocking peptide
[Similar Products]
Product Synonym Gene Name
C2F; NEP1; Grcc2f[Similar Products]
EMG1 peptide (MBS3230343) is used for blocking the activity of EMG1 antibody (MBS3205377)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q92979
Form/Format
Lyophilized powder
Preparation and Storage
Add 100ul of sterile PBS. Final peptide concentration is 1 mg/ml in PBS. For longer periods of storage, store at -20 degree C. Avoid repeat freeze-thaw cycles.
Other Notes
Small volumes of EMG1 blocking peptide vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
EMG1 blocking peptide
This is a synthetic peptide designed for use in combination with anti-EMG1 antibody made
Target Description: EMG1 involved in 40S ribosomal subunit biogenesis. It seems to play a role a methylation reaction in pre-rRNA processing.
Product Categories/Family for EMG1 blocking peptide
Peptide
Applications Tested/Suitable for EMG1 blocking peptide
Immunohistochemistry (IHC), Western Blot (WB)
NCBI/Uniprot data below describe general gene information for EMG1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_006322
[Other Products]
NCBI GenBank Nucleotide #
NM_006331
[Other Products]
UniProt Primary Accession #
Q92979
[Other Products]
UniProt Related Accession #
Q92979[Other Products]
NCBI Official Full Name
ribosomal RNA small subunit methyltransferase NEP1 isoform 1
NCBI Official Synonym Full Names
EMG1 N1-specific pseudouridine methyltransferase
NCBI Official Symbol
EMG1??[Similar Products]
NCBI Official Synonym Symbols
C2F; NEP1; Grcc2f
??[Similar Products]
NCBI Protein Information
ribosomal RNA small subunit methyltransferase NEP1
UniProt Protein Name
Ribosomal RNA small subunit methyltransferase NEP1
UniProt Synonym Protein Names
18S rRNA (pseudouridine(1248)-N1)-methyltransferase; 18S rRNA Psi1248 methyltransferase; Nucleolar protein EMG1 homolog; Protein C2f; Ribosome biogenesis protein NEP1
UniProt Gene Name
EMG1??[Similar Products]
UniProt Entry Name
NEP1_HUMAN
NCBI Summary for EMG1
This gene encodes an essential, conserved eukaryotic protein that methylates pseudouridine in 18S rRNA. The related protein in yeast is a component of the small subunit processome and is essential for biogenesis of the ribosomal 40S subunit. A mutation in this gene has been associated with Bowen-Conradi syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
UniProt Comments for EMG1
C2F: Involved in 40S ribosomal subunit biogenesis and 18S rRNA processing. Specifically catalyzes the N1-methylation of pseudouridine at position 1248 (Psi1248) in 18S rRNA. Thus, appears to be the methyltransferase involved in the biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) in position 1248 in 18S rRNA. Is not able to methylate uridine at this position. Defects in EMG1 are the cause of Bowen-Conradi syndrome (BWCNS). BWCNS is a combination of malformations characterized in newborns by low birth weight, microcephaly, mild joint restriction, a prominent nose, micrognathia, fifth finger clinodactyly, and 'rocker-bottom' feet. The syndrome is transmitted as an autosomal recessive trait. The prognosis is poor, with all infants dying within the first few months of life. Belongs to the NEP1 family.
Protein type: RNA-binding; EC 2.1.1.-; Nucleolus
Chromosomal Location of Human Ortholog: 12p13.3
Cellular Component: cytoplasm; nucleolus; nucleus
Molecular Function: rRNA binding; RNA binding
Biological Process: ribosomal small subunit biogenesis and assembly; rRNA methylation; rRNA processing
Disease: Bowen-conradi Syndrome
Research Articles on EMG1
1. Our findings further indicate that in Bowen-Conradi syndrome, nuclear disassembly of the import complex and release of EMG1D86G lead to its nuclear aggregation and degradation, resulting in the reduced nucleolar recruitment of the RNA methyltransferase and defects in the biogenesis of the small ribosomal subunit.
Precautions
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Disclaimer
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