Product Name
EMG1, Polyclonal Antibody
Popular Item
Full Product Name
EMG1 Polyclonal Antibody
Product Synonym Names
C2F; NEP1; Grcc2f
Product Gene Name
anti-EMG1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q92979
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human EMG1
Calculated Molecular Weight
27kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Other Notes
Small volumes of anti-EMG1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-EMG1 antibody
This gene encodes an essential, conserved eukaryotic protein involved in ribosome biogenesis. In yeast, the related protein is a component of the small subunit processome and is essential for biogenesis of the ribosomal 40S subunit. A mutation in this gene has been associated with Bowen-Conradi syndrome.
Product Categories/Family for anti-EMG1 antibody
Polyclonal
Applications Tested/Suitable for anti-EMG1 antibody
Western Blot (WB)
Application Notes for anti-EMG1 antibody
WB: 1:500 - 1:2000
Western Blot (WB) of anti-EMG1 antibody
Western blot analysis of extracts of various cell lines, using EMG1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
NCBI/Uniprot data below describe general gene information for EMG1. It may not necessarily be applicable to this product.
NCBI Accession #
Q92979.4
[Other Products]
UniProt Primary Accession #
Q92979
[Other Products]
UniProt Secondary Accession #
O00675; O00726[Other Products]
UniProt Related Accession #
Q92979[Other Products]
NCBI Official Full Name
Ribosomal RNA small subunit methyltransferase NEP1
NCBI Official Synonym Full Names
EMG1 N1-specific pseudouridine methyltransferase
NCBI Official Symbol
EMG1??[Similar Products]
NCBI Official Synonym Symbols
C2F; NEP1; Grcc2f
??[Similar Products]
NCBI Protein Information
ribosomal RNA small subunit methyltransferase NEP1; EMG1 nucleolar protein homolog; essential for mitotic growth 1; ribosome biogenesis protein NEP1; 18S rRNA Psi1248 methyltransferase; 18S rRNA (pseudouridine(1248)-N1)-methyltransferase; 18S rRNA (pseudouridine-N1-)-methyltransferase NEP1
UniProt Protein Name
Ribosomal RNA small subunit methyltransferase NEP1
UniProt Synonym Protein Names
18S rRNA (pseudouridine(1248)-N1)-methyltransferase; 18S rRNA Psi1248 methyltransferase; Nucleolar protein EMG1 homolog; Protein C2f; Ribosome biogenesis protein NEP1
UniProt Gene Name
EMG1??[Similar Products]
UniProt Entry Name
NEP1_HUMAN
NCBI Summary for EMG1
This gene encodes an essential, conserved eukaryotic protein involved in ribosome biogenesis. In yeast, the related protein is a component of the small subunit processome and is essential for biogenesis of the ribosomal 40S subunit. A mutation in this gene has been associated with Bowen-Conradi syndrome.[provided by RefSeq, May 2010]
UniProt Comments for EMG1
C2F: Involved in 40S ribosomal subunit biogenesis and 18S rRNA processing. Specifically catalyzes the N1-methylation of pseudouridine at position 1248 (Psi1248) in 18S rRNA. Thus, appears to be the methyltransferase involved in the biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) in position 1248 in 18S rRNA. Is not able to methylate uridine at this position. Defects in EMG1 are the cause of Bowen-Conradi syndrome (BWCNS). BWCNS is a combination of malformations characterized in newborns by low birth weight, microcephaly, mild joint restriction, a prominent nose, micrognathia, fifth finger clinodactyly, and 'rocker-bottom' feet. The syndrome is transmitted as an autosomal recessive trait. The prognosis is poor, with all infants dying within the first few months of life. Belongs to the NEP1 family.
Protein type: EC 2.1.1.-; RNA-binding; Nucleolus
Chromosomal Location of Human Ortholog: 12p13.3
Cellular Component: cytoplasm; nucleolus; nucleus
Molecular Function: rRNA binding; RNA binding
Biological Process: ribosomal small subunit biogenesis and assembly; rRNA methylation; rRNA processing
Disease: Bowen-conradi Syndrome
Research Articles on EMG1
1. Mutation of a gene essential for ribosome biogenesis, EMG1, causes Bowen-Conradi syndrome.
Precautions
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