Full Product Name
EMG1 antibody - N-terminal region
Product Gene Name
anti-EMG1 antibody
[Similar Products]
Product Synonym Gene Name
C2F; NEP1; Grcc2f[Similar Products]
Antibody/Peptide Pairs
EMG1 peptide (MBS3230343) is used for blocking the activity of EMG1 antibody (MBS3205377)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
Synthetic peptide located within the following region: SLETVKVGKT YELLNCDKHK SILLKNGRDP GEARPDITHQ SLLMLMDSPL
3D Structure
ModBase 3D Structure for Q92979
Species Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Purity/Purification
Affinity Purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Homology
Cow: 92%; Dog: 92%; Guinea Pig: 92%; Horse: 92%; Human: 100%; Mouse: 93%; Rabbit: 92%; Rat: 93%; Zebrafish: 79%
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human EMG1
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-EMG1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-EMG1 antibody
This is a rabbit polyclonal antibody against EMG1. It was validated on Western Blot and immunohistochemistry
Target Description: EMG1 involved in 40S ribosomal subunit biogenesis. It seems to play a role a methylation reaction in pre-rRNA processing.
Product Categories/Family for anti-EMG1 antibody
Polyclonal; RNA Binding Proteins; Immunohistochemistry;
Applications Tested/Suitable for anti-EMG1 antibody
Immunohistochemistry (IHC), Western Blot (WB)
Immunohistochemistry (IHC) of anti-EMG1 antibody
Rabbit Anti-EMG1 Antibody
Paraffin Embedded Tissue: Human Kidney
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X
Western Blot (WB) of anti-EMG1 antibody
WB Suggested Anti-EMG1 Antibody Titration: 0.2-1 ug/ml
Positive Control: HepG2 cell lysateEMG1 is supported by BioGPS gene expression data to be expressed in HepG2
NCBI/Uniprot data below describe general gene information for EMG1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_006322
[Other Products]
NCBI GenBank Nucleotide #
NM_006331
[Other Products]
UniProt Primary Accession #
Q92979
[Other Products]
UniProt Related Accession #
Q92979[Other Products]
NCBI Official Full Name
ribosomal RNA small subunit methyltransferase NEP1 isoform 1
NCBI Official Synonym Full Names
EMG1 N1-specific pseudouridine methyltransferase
NCBI Official Symbol
EMG1??[Similar Products]
NCBI Official Synonym Symbols
C2F; NEP1; Grcc2f
??[Similar Products]
NCBI Protein Information
ribosomal RNA small subunit methyltransferase NEP1
UniProt Protein Name
Ribosomal RNA small subunit methyltransferase NEP1
UniProt Synonym Protein Names
18S rRNA (pseudouridine(1248)-N1)-methyltransferase; 18S rRNA Psi1248 methyltransferase; Nucleolar protein EMG1 homolog; Protein C2f; Ribosome biogenesis protein NEP1
UniProt Gene Name
EMG1??[Similar Products]
UniProt Entry Name
NEP1_HUMAN
NCBI Summary for EMG1
This gene encodes an essential, conserved eukaryotic protein that methylates pseudouridine in 18S rRNA. The related protein in yeast is a component of the small subunit processome and is essential for biogenesis of the ribosomal 40S subunit. A mutation in this gene has been associated with Bowen-Conradi syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
UniProt Comments for EMG1
C2F: Involved in 40S ribosomal subunit biogenesis and 18S rRNA processing. Specifically catalyzes the N1-methylation of pseudouridine at position 1248 (Psi1248) in 18S rRNA. Thus, appears to be the methyltransferase involved in the biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) in position 1248 in 18S rRNA. Is not able to methylate uridine at this position. Defects in EMG1 are the cause of Bowen-Conradi syndrome (BWCNS). BWCNS is a combination of malformations characterized in newborns by low birth weight, microcephaly, mild joint restriction, a prominent nose, micrognathia, fifth finger clinodactyly, and 'rocker-bottom' feet. The syndrome is transmitted as an autosomal recessive trait. The prognosis is poor, with all infants dying within the first few months of life. Belongs to the NEP1 family.
Protein type: RNA-binding; EC 2.1.1.-; Nucleolus
Chromosomal Location of Human Ortholog: 12p13.3
Cellular Component: cytoplasm; nucleolus; nucleus
Molecular Function: rRNA binding; RNA binding
Biological Process: ribosomal small subunit biogenesis and assembly; rRNA methylation; rRNA processing
Disease: Bowen-conradi Syndrome
Research Articles on EMG1
1. Our findings further indicate that in Bowen-Conradi syndrome, nuclear disassembly of the import complex and release of EMG1D86G lead to its nuclear aggregation and degradation, resulting in the reduced nucleolar recruitment of the RNA methyltransferase and defects in the biogenesis of the small ribosomal subunit.
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