Human Carboxypeptidase N catalytic chain (CPN1) ELISA kit; CPN; FLJ40792; SCPN; anaphylatoxin inactivator; arginine carboxypeptidase; carboxypeptidase N catalytic subunit; carboxypeptidase N polypeptide 1 50 kD; carboxypeptidase N small subunit; kininase I; lysin; carboxypeptidase N; polypeptide 1

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of human CPN1. No significant cross-reactivity or interference between human CPN1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of CPN1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS946937 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the carboxypeptidase N, polypeptide 1 (CPN1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CPN1. The ELISA analytical biochemical technique of the MBS946937 kit is based on CPN1 antibody-CPN1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CPN1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CPN1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CPN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CPN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CPN1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CPN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

52,286 Da

carboxypeptidase N, polypeptide 1

carboxypeptidase N catalytic chain; kininase I; kininase-1; carboxypeptidase K; lysine carboxypeptidase; serum carboxypeptidase N; anaphylatoxin inactivator; arginine carboxypeptidase; plasma carboxypeptidase B; carboxypeptidase N small subunit; carboxypeptidase N catalytic subunit; carboxypeptidase N polypeptide 1 50 kD

Carboxypeptidase N catalytic chain

ACBP; CPN; SCPN??[Similar Products]

CBPN_HUMAN

Carboxypeptidase N is a plasma metallo-protease that cleaves basic amino acids from the C terminal of peptides and proteins. The enzyme is important in the regulation of peptides like kinins and anaphylatoxins, and has also been known as kininase-1 and anaphylatoxin inactivator. This enzyme is a tetramer comprised of two identical regulatory subunits and two identical catalytic subunits; this gene encodes the catalytic subunit. Mutations in this gene can be associated with angioedema or chronic urticaria resulting from carboxypeptidase N deficiency. [provided by RefSeq, Jul 2008]

CPN1: Protects the body from potent vasoactive and inflammatory peptides containing C-terminal Arg or Lys (such as kinins or anaphylatoxins) which are released into the circulation. Defects in CPN1 are the cause of carboxypeptidase N deficiency (CPND). Patients affected present some combination of angioedema or chronic urticaria, as well as hay fever or astma, and have also slightly depressed serum carboxy peptidase N, suggestive of autosomal recessive inheritance of this disorder. Belongs to the peptidase M14 family.

Protein type: EC 3.4.17.3; Secreted; Protease; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q24.2

Cellular Component: extracellular space

Molecular Function: zinc ion binding; metallocarboxypeptidase activity

Biological Process: response to glucocorticoid stimulus; proteolysis

Disease: Carboxypeptidase N Deficiency

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