For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of NPW elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7240801 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Neuropeptide W (NPW) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NPW. The ELISA analytical biochemical technique of the MBS7240801 kit is based on NPW antibody-NPW antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NPW antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NPW. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Neuroscience

18,048 Da

neuropeptide W

neuropeptide W; hPPL8; preproprotein L8; prepro-neuropeptide W

Neuropeptide W

PPL8; PPNPW; hPPL8; NPW23; hL8; NPW30; hL8C??[Similar Products]

NPW_HUMAN

The product of this gene is processed into 23- and 30-amino acid neuropeptides that bind and activate two G-protein coupled receptors in the central nervous system. The neuropeptides have been shown to enhance cortisol secretion from adrenal cells through the adenylate cyclase/protein kinase A signaling cascade. The preproprotein is translated using a non-AUG initiation codon that is inferred from analyses of the mouse ortholog. [provided by RefSeq, Jul 2008]

NPW: Plays a regulatory role in the organization of neuroendocrine signals accessing the anterior pituitary gland. Stimulates water drinking and food intake. May play a role in the hypothalamic response to stress. NPW23 activates GPR7 and GPR8 more efficiently than NPW30. Belongs to the neuropeptide B/W family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: extracellular region

Molecular Function: protein binding; G-protein-coupled receptor binding

Biological Process: G-protein coupled receptor protein signaling pathway; neuropeptide signaling pathway; feeding behavior

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