Rat Neuropeptide W (NP-W) ELISA Kit; L8; L8C; PPL8; PPNPW; ; neuropeptide W

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of rat NP-W. No significant cross-reactivity or interference between rat NP-W and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of NPW elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS706200 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the neuropeptide W (NPW) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NPW. The ELISA analytical biochemical technique of the MBS706200 kit is based on NPW antibody-NPW antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NPW antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NPW. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NP-W has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NP-W present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NP-W is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NP-W bound in the initial step. The color development is stopped and the intensity of the color is measured.

20,231 Da

neuropeptide W

neuropeptide W; PPL8; preproprotein L8; prepro-Neuropeptide W polypeptide

Neuropeptide W

Ppl8; PPL8; NPW23; NPW30??[Similar Products]

NPW_RAT

endogenous ligand for both GPR7 and GPR8; involved in the central control of feeding [RGD, Feb 2006]

Function: Plays a regulatory role in the organization of neuroendocrine signals accessing the anterior pituitary gland. Stimulates water drinking and food intake. May play a role in the hypothalamic response to stress. When injected into the lateral cerebroventricle, it elevates prolactin (PRL) and corticosterone and lowers growth hormone (GH) release.1 PublicationManual assertion based on experiment in:Ref.2

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