Product Name
VAMP-1,2/Synaptobrevin (VAMP1), Monoclonal Antibody
Full Product Name
Anti-VAMP-1,2/Synaptobrevin Monoclonal Antibody
Product Gene Name
anti-VAMP1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 12; NC_000012.11 (6571403..6579843, complement). Location: 12p
3D Structure
ModBase 3D Structure for P23763
Specificity
VAMP-1,2/Synaptobrevin
This antibody recognizes human VAMP1 and VAMP2.
Form/Format
100ug in PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.
Antigen
Crude human synaptic immunoprecipitate.
Dilution Instructions
Dilute in PBS or medium which is identical to that used in the assay system.
Preparation and Storage
This antibody is stable for at least one (1) year at -20 degree C. Avoid repeated freezing and thawing.
Other Notes
Small volumes of anti-VAMP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-VAMP1 antibody
VAMP-1 (Vesicle-Associated Membrane Protein) is an 18kDa member of the synaptobrevin family. It is expressed in neurons, neutrophils, and skeletal muscle cells and participates in vesicle fusion with the plasma membrane. Synaptobrevins, syntaxins, SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. VAMP-1 is also one of the key proteins in the SNARE complex involved in regulated exocytosis.
Applications Tested/Suitable for anti-VAMP1 antibody
Immunoblot
Application Notes for anti-VAMP1 antibody
Immunoblotting: use at 0.5-1.0ug/ml. A band of ~18kDa is detected. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Human brain lysate
NCBI/Uniprot data below describe general gene information for VAMP1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_954740.1
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NCBI GenBank Nucleotide #
NM_199245.1
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UniProt Primary Accession #
P23763
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UniProt Secondary Accession #
O75468; Q15857; Q6FG94; Q8IVC9; A8MVP3; D3DUR3[Other Products]
UniProt Related Accession #
P23763[Other Products]
Molecular Weight
12,902 Da[Similar Products]
NCBI Official Full Name
vesicle-associated membrane protein 1 isoform 2
NCBI Official Synonym Full Names
vesicle-associated membrane protein 1 (synaptobrevin 1)
NCBI Official Symbol
VAMP1??[Similar Products]
NCBI Official Synonym Symbols
SYB1; VAMP-1; DKFZp686H12131
??[Similar Products]
NCBI Protein Information
vesicle-associated membrane protein 1; synaptobrevin 1; synaptobrevin-1; OTTHUMP00000238453; OTTHUMP00000238454; OTTHUMP00000238455; OTTHUMP00000238560; OTTHUMP00000238561
UniProt Protein Name
Vesicle-associated membrane protein 1
UniProt Synonym Protein Names
Synaptobrevin-1
Protein Family
Vesicle-associated membrane protein
UniProt Gene Name
VAMP1??[Similar Products]
UniProt Synonym Gene Names
SYB1??[Similar Products]
UniProt Entry Name
VAMP1_HUMAN
NCBI Summary for VAMP1
Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. VAMP1 is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Multiple alternative splice variants that encode proteins with alternative carboxy ends have been described, but the full-length nature of some variants has not been defined. [provided by RefSeq]
UniProt Comments for VAMP1
VAMP1: Involved in the targeting and/or fusion of transport vesicles to their target membrane. Belongs to the synaptobrevin family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 12p
Cellular Component: SNARE complex; mitochondrial outer membrane; synaptic vesicle; neuron projection; cell surface; synaptic vesicle membrane; integral to plasma membrane; cytosol; cell junction
Molecular Function: SNAP receptor activity; SNARE binding; protein binding
Biological Process: vesicle fusion; exocytosis; pathogenesis
Disease: Spastic Ataxia 1, Autosomal Dominant
Research Articles on VAMP1
1. multiple domains outside the R-SNARE of tomosyn are critical to the efficacy of inhibition by tomosyn on exocytotic secretion
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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