Product Name
VAMP 1 (Vamp1), Monoclonal Antibody
Full Product Name
VAMP 1 (Vesicle Associated Membrane Protein 1, Vesicle-associated Membrane Protein 1, VAMP1, VAMP-1, DKFZp686H12131, Synaptobrevin 1, Synaptobrevin-1, SYB1, SYB-1)
Product Synonym Names
Anti -VAMP 1 (Vesicle Associated Membrane Protein 1, Vesicle-associated Membrane Protein 1, VAMP1, VAMP-1, DKFZp686H12131, Synaptobrevin 1, Synaptobrevin-1, SYB1, SYB-1)
Product Gene Name
anti-Vamp1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 4; NC_005103.2 (161333661..161340447). Location: 4q42
3D Structure
ModBase 3D Structure for Q63666
Species Reactivity
Human, Mouse
Specificity
Recognizes endogenous human VAMP-1 and VAMP-2 at ~16kD. Does not react with recombinant VAMP family members 5, 7, 8. Species Crossreactivity: mouse.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS.
Immunogen
E. coli-derived recombinant human VAMP-1 (aa1-96; Accession # P23763).
Hybridoma
Mouse myeloma cells with B cells obtained from a mouse immunized with purified, E. coli-derived, recombinant human VAMP-1 (aa1-96).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-Vamp1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Vamp1 antibody
VAMP-1 (vesicle-associated membrane protein 1; also synaptobrevin-1/SYB1) is an 18kD member of the synaptobrevin family of proteins. It is expressed in neurons, neutrophils and skeletal muscle cells, and participates in vesicle fusion with the plasma membrane. Human VAMP-1 is a type IV transmembrane protein that contains an N-terminal cytoplasmic region (aa1-96) and a 22aa transmembrane domain (aa97-118). There is one coiled-coil region between aa33-93. Multiple splice variants are known that show two, three, four and 81aa substitutions for the C-terminal five amino acids. One three aa variant creates a mitochondrial targeting motif. over aa1-96, human VAMP-1 is 98% aa identical to mouse VAMP-1.
Product Categories/Family for anti-Vamp1 antibody
Antibodies; Abs to Membrane Proteins
Applications Tested/Suitable for anti-Vamp1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-Vamp1 antibody
Suitable for use in Western Blot and Immunohistochemistry.
Dilution: Western Blot: 0.2ug/ml
Immunohistochemistry: 25ug/ml
NCBI/Uniprot data below describe general gene information for Vamp1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_037222.2
[Other Products]
NCBI GenBank Nucleotide #
NM_013090.2
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UniProt Primary Accession #
Q63666
[Other Products]
UniProt Secondary Accession #
O09025; Q56A22; Q8CH14; A6YSN3[Other Products]
UniProt Related Accession #
Q63666[Other Products]
Molecular Weight
12,797 Da[Similar Products]
NCBI Official Full Name
vesicle-associated membrane protein 1
NCBI Official Synonym Full Names
vesicle-associated membrane protein 1
NCBI Official Symbol
Vamp1??[Similar Products]
NCBI Official Synonym Symbols
Syb1
??[Similar Products]
NCBI Protein Information
vesicle-associated membrane protein 1; VAMP-1; synaptobrevin 1; synaptobrevin-1
UniProt Protein Name
Vesicle-associated membrane protein 1
UniProt Synonym Protein Names
Synaptobrevin-1
Protein Family
Vesicle-associated membrane protein
UniProt Gene Name
Vamp1??[Similar Products]
UniProt Synonym Gene Names
Syb1??[Similar Products]
UniProt Entry Name
VAMP1_RAT
NCBI Summary for Vamp1
plays a role in protein transport to the plasma membrane [RGD]
UniProt Comments for Vamp1
VAMP1: Involved in the targeting and/or fusion of transport vesicles to their target membrane. Belongs to the synaptobrevin family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Cellular Component: SNARE complex; mitochondrial outer membrane; synaptic vesicle; cell surface; cytoplasmic vesicle membrane; synaptic vesicle membrane; mitochondrion; integral to membrane; terminal button; cytosol; cell junction
Molecular Function: SNAP receptor activity; protein binding; SNARE binding
Biological Process: vesicle-mediated transport; vesicle fusion; intracellular protein transport; exocytosis; neurotransmitter secretion
Research Articles on Vamp1
1. multiple domains outside the R-SNARE of tomosyn are critical to the efficacy of inhibition by tomosyn on exocytotic secretion
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
