Anti -Solute Carrier Family 12a1 (Slc12a1, sodium/potassium/chloride transporters, member 1, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, Kidney-specific Na-K-Cl symporter, MGC93479, Nkcc2, Solute carrier family 12 member 1)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 15; NC_000015.9 (48498498..48596275). Location: 15q15-q21.1

Polyclonal

IgG

Rabbit

Recognizes rat Slc12a1. Species Crossreactivity: Mouse, human

Serum
Serum

Supplied as a liquid, 50% glycerol.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Small volumes of anti-SLC12A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Slc12a1, a kidney-specific member of the cation chloride co-transporter family SLC12, facilitates transport of sodium, potassium, and chloride ions from the lumen of the renal thick ascending limb into the cell (1, 2). Transport of sodium dilutes the luminal fluid, decreasing its osmolality creating an osmotic driving force for water reabsorption in the connecting tubule and cortical

Antibodies; Abs to Ion Channel

Western Blot (WB)

Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000

121,450 Da[Similar Products]

solute carrier family 12 (sodium/potassium/chloride transporters), member 1

solute carrier family 12 member 1; NKCC2A variant A; OTTHUMP00000162220; Na-K-2Cl cotransporter; kidney-specific Na-K-Cl symporter; bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2

Solute carrier family 12 member 1

NKCC2??[Similar Products]

S12A1_HUMAN

This gene encodes a kidney-specific sodium-potassium-chloride cotransporter that is expressed on the luminal membrane of renal epithelial cells of the thick ascending limb of Henle's loop and the macula densa. It plays a key role in concentrating urine and accounts for most of the NaCl resorption. It is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their biological validity in humans has not been experimentally proven.

NKCC2: Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume. Kidney specific. Activated by WNK3. Belongs to the SLC12A transporter family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter; Membrane protein, multi-pass; Membrane protein, integral; Transporter, SLC family

Chromosomal Location of Human Ortholog: 15q15-q21.1

Cellular Component: membrane; apical plasma membrane; integral to membrane; plasma membrane

Molecular Function: sodium:potassium:chloride symporter activity

Biological Process: transport; sodium ion transport; chloride transport; ion transport; kidney development; excretion; transmembrane transport; potassium ion transport; chemical homeostasis

Disease: Bartter Syndrome, Antenatal, Type 1

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