Full Product Name
Anti-AQP1 Rabbit Monoclonal Antibody
Product Synonym Names
Aquaporin-1; AQP-1; Aquaporin-CHIP; Urine water channel; Water channel protein for red blood cells and kidney proximal tubule; AQP1; CHIP28
Product Gene Name
anti-AQP1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P29972
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity-chromatography
Immunogen
A synthesized peptide derived from human AQP1
Subcellular Localization
Cell membrane; Multi-pass membrane protein.
Tissue Specificity
Detected in erythrocytes (at protein level). Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of anti-AQP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-AQP1 antibody
Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
Product Categories/Family for anti-AQP1 antibody
Signal Transduction; Metabolism; plasma membrane; channels; Signal Transduction; vitamins/minerals
Applications Tested/Suitable for anti-AQP1 antibody
Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-AQP1 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
Western Blot (WB) of anti-AQP1 antibody
Western blot analysis of AQP1 expression in human fetal kidney lysate (MBS179477).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AQP1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for AQP1
NCBI/Uniprot data below describe general gene information for AQP1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_932766.1
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NCBI GenBank Nucleotide #
NM_198098.3
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UniProt Primary Accession #
P29972
[Other Products]
UniProt Secondary Accession #
Q8TBI5; Q8TDC1; B5BU39; E7EM69; E9PC21; F5GY19[Other Products]
UniProt Related Accession #
P29972[Other Products]
Molecular Weight
28526 MW
NCBI Official Full Name
aquaporin-1 isoform 1
NCBI Official Synonym Full Names
aquaporin 1 (Colton blood group)
NCBI Official Symbol
AQP1??[Similar Products]
NCBI Official Synonym Symbols
CO; CHIP28; AQP-CHIP
??[Similar Products]
NCBI Protein Information
aquaporin-1
UniProt Protein Name
Aquaporin-1
UniProt Synonym Protein Names
Aquaporin-CHIP; Urine water channel; Water channel protein for red blood cells and kidney proximal tubule
UniProt Gene Name
AQP1??[Similar Products]
UniProt Synonym Gene Names
CHIP28; AQP-1??[Similar Products]
NCBI Summary for AQP1
This gene encodes a small integral membrane protein with six bilayer spanning domains that functions as a water channel protein. This protein permits passive transport of water along an osmotic gradient. This gene is a possible candidate for disorders involving imbalance in ocular fluid movement. [provided by RefSeq, Aug 2016]
UniProt Comments for AQP1
Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
Research Articles on AQP1
1. The BRAF V600 mutations were significantly associated with AQP1 expression (P=0.014). Long-term follow-up indicated a reduced progression-free survival (P=0.036) and overall survival (P=0.017) for the AQP1-positive cutaneous melanoma patients.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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