For Research Use Only. Not for use in diagnostic procedures.

This kit recognizes natural and recombinant Monkey AQP-1. No significant cross-reactivity or interference between Monkey AQP-1 and analogues was observed.

Store at 4 degree C.

Manufactured in an ISO 9001:2015 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of AQP-1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2514905 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the AQP-1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AQP-1. The ELISA analytical biochemical technique of the MBS2514905 kit is based on AQP-1 antibody-AQP-1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AQP-1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AQP-1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Monkey AQP-1 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to AQP-1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for AQP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain AQP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of AQP-1. You can calculate the concentration of AQP-1 in the samples by comparing the OD of the samples to the standard curve.

28,793 Da

aquaporin 1

aquaporin-1; AQP-1; DER2; aquaporin-CHIP; delayed early response protein 2; growth factor-induced delayed early response protein; water channel protein for red blood cells and kidney proximal tubule

Aquaporin-1

AQP-1; DER2??[Similar Products]

AQP1_MOUSE

AQP1: Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Belongs to the MIP/aquaporin (TC 1.A.8) family.

Protein type: Transporter, aquaporin family; Membrane protein, multi-pass; Membrane protein, integral; Transporter

Cellular Component: nuclear membrane; neuron projection; brush border membrane; cell; basolateral plasma membrane; integral to plasma membrane; integral to membrane; dense core granule membrane; membrane; axon; apical part of cell; cytoplasm; apical plasma membrane; plasma membrane; basal plasma membrane; nerve terminal; nucleus; sarcolemma; brush border

Molecular Function: potassium channel activity; ephrin receptor binding; water transporter activity; transporter activity; ammonium transmembrane transporter activity; intracellular cGMP activated cation channel activity; potassium ion transmembrane transporter activity; glycerol transmembrane transporter activity; nitric oxide transporter activity; water channel activity; transmembrane transporter activity; glycerol channel activity

Biological Process: cGMP biosynthetic process; glycerol transport; wound healing; water transport; carbon dioxide transport; cellular response to stress; negative regulation of caspase activity; sensory perception of pain; positive regulation of saliva secretion; water homeostasis; positive regulation of fibroblast proliferation; organic cation transport; nitric oxide transport; transport; cellular homeostasis; transmembrane transport; lipid digestion; potassium ion transport; secretion by cell; response to drug; cellular water homeostasis; secretory granule organization and biogenesis; glomerular filtration; ammonium transport; positive regulation of angiogenesis; renal water transport; camera-type eye morphogenesis; adrenocorticotropin hormone secretion; establishment and/or maintenance of actin cytoskeleton polarity; cell volume homeostasis; positive regulation of cell migration; negative regulation of apoptosis

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