Glutamate Transporter EAAT4 (Excitatory Amino Acid Transporter 4)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 20; NC_006602.2 (50137157..50147431)

Polyclonal

IgG

Host: Rabbit
Source: Rat

The peptide was found unique to EAAT4 without significant homology to other glutamate transporters. It is 100% homologous in mouse and 90% in human EAAT4. Antibody crossreactivity in various species is not established.

Serum

Serum
Supplied as a liquid, neat serum, 40% glycerol.

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-SLC1A6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Glutamate is the main excitatory neurotransmitter in the brain. To date five glutamate Transporters have been cloned: GLAST (EAAT1), GLT1 (EAAT2), EAAC1 (EAAT3), EAAT4, and EAAT5. These transporters are believed to be critical in reducing potentially toxic extracellular concentration of glutamate by rapid uptake into nerve terminals and glial cells. Glutamate transporters (525-573aa) display about 55% homology and are predicted to contain up to 6-10 transmembrane domains. Immunolocalization studies indicate that GLT1 is localized in astroglial cells throughout the brain and spinal cord. EAAC1 is specific for certain neurons and purkinje cells, and specifically enriched in cortex, hippocampus, and caudate-putamen and confined to presynaptic and postsynaptic elements. GLAST has been observed in both neuron and astroglia. It is most abundant in Bergmann glia, cortex, hippocampus and cerebellum. EAAT4 has properties of ligand gated Cl-channel. It is localized mainly in cerebellar Purkinje cells in rat and human CNS. EAAT5 has only been cloned from human. It is primarily expressed in retina.

Abs to Proteins

ELISA, Western Blot

Suitable for use in ELISA, Western Blotting. Other applications not tested.
Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using Chemiluminescence technique (2). EAAT4 monomers in freshly made rat brain extracts appeared as fuzzy ~65kD protein (2). High MW bands may be see after oxidation (2).
ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure. Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Control peptide, because of its low MW (<3kD), is not suitable for Western. It is recommeded for ELISA, dot blot, or for antibody blocking experiments (use 5-10ug of control peptide per 1ug of aff pure IgG or 1ul of antiserum) to demonstrate antibody specificity

61,466 Da[Similar Products]

excitatory amino acid transporter 4; glutamate transporter; solute carrier family 1 member 6; sodium-dependent glutamate/aspartate transporter

Excitatory amino acid transporter 4

EAAT4??[Similar Products]

EAA4_CANFA

??1. Lin C et al (1997) gene accession# g3023680; Maeno-Hikichi Y et al (1997) Brain Res. Mol. Brain. Res. 48, 176-180; Fairbanks WA et a (1995) Nature 375, 599-6032. Dehnes Y et al (1998) J Neurosci. 18, 3606-3619; Furuta A et al (1997) J Neurosci. 17, 8363-8375.

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.