Amnionless (AMN)

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Mouse AMN. No significant cross-reactivity or interference between Mouse AMN and analogues was observed.

Store at 2-8 degree C.

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Small volumes of AMN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS285448 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Protein amnionless, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AMN. The ELISA analytical biochemical technique of the MBS285448 kit is based on AMN antibody-AMN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AMN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AMN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate AMN in samples. An antibody specific for AMN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAMN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AMN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AMN bound in the initial step. The color development is stopped and the intensity of the color is measured.

48,696 Da

amnionless

protein amnionless

Protein amnionless

This gene encodes a type I transmembrane protein. The encoded protein is an essential component of the cubulin receptor complex which is thought to play a role in coordinating growth and patterning of the embryo. This protein is thought to modulate a bone morphogenetic protein (BMP) signaling pathway. A homoygous mutation in the mouse gene results in the lack of an amnion in embryos. Mutations in the human gene are associated with Megaloblastic Anemia-1. [provided by RefSeq, Sep 2015]

AMN: Necessary for efficient absorption of vitamin B12. May direct the production of trunk mesoderm during development by modulating a bone morphogenetic protein (BMP) signaling pathway in the underlying visceral endoderm. Defects in AMN are a cause of recessive hereditary megaloblastic anemia 1 (RH-MGA1); also known as MGA1 Norwegian type or Imerslund-Grasbeck syndrome (I-GS). RH-MGA1 is due to selective malabsorption of vitamin B12. Defects in vitamin B12 absorption lead to impaired function of thymidine synthase. As a consequence DNA synthesis is interrupted. Rapidly dividing cells involved in erythropoiesis are particularly affected. 1 isoforms of the human protein are produced by alternative promoter.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12 F1|12 60.94 cM

Cellular Component: apical part of cell; apical plasma membrane; brush border membrane; clathrin-coated pit; endocytic vesicle; endosome; extracellular space; integral component of membrane; membrane; plasma membrane; protein complex

Molecular Function: receptor binding

Biological Process: cobalamin transport; excretion; Golgi to plasma membrane protein transport; multicellular organism development; protein localization; protein transport; receptor-mediated endocytosis; transport

Disease: Megaloblastic Anemia 1

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