Mouse Cubilin (CUBN) ELISA kit; RP11-416D8.1; FLJ90055; FLJ90747; IFCR; MGA1; gp280; cubilin; intrinsic factor B12-receptor; intrinsic factor-cobalamin receptor; intrinsic factor-vitamin B12 receptor; cubilin (intrinsic factor-cobalamin receptor)

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of mouse CUBN. No significant cross-reactivity or interference between mouse CUBN and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of CUBN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS939514 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cubilin (intrinsic factor-cobalamin receptor) (CUBN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CUBN. The ELISA analytical biochemical technique of the MBS939514 kit is based on CUBN antibody-CUBN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CUBN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CUBN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CUBN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CUBN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CUBN is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CUBN bound in the initial step. The color development is stopped and the intensity of the color is measured.

399,097 Da

cubilin (intrinsic factor-cobalamin receptor)

cubilin; intrinsic factor-cobalamin receptor

Cubilin

Ifcr??[Similar Products]

CUBN_MOUSE

CUBN: Cotransporter which plays a role in lipoprotein, vitamin and iron metabolism, by facilitating their uptake. Binds to ALB, MB, Kappa and lambda-light chains, TF, hemoglobin, GC, SCGB1A1, APOA1, high density lipoprotein, and the GIF-cobalamin complex. The binding of all ligands requires calcium. Serves as important transporter in several absorptive epithelia, including intestine, renal proximal tubules and embryonic yolk sac. Interaction with LRP2 mediates its trafficking throughout vesicles and facilitates the uptake of specific ligands like GC, hemoglobin, ALB, TF and SCGB1A1. Interaction with AMN controls its trafficking to the plasma membrane and facilitates endocytosis of ligands. May play an important role in the development of the peri-implantation embryo through internalization of APOA1 and cholesterol. Binds to LGALS3 at the maternal-fetal interface. Defects in CUBN are a cause of recessive hereditary megaloblastic anemia 1 (RH-MGA1); also known as MGA1 Norwegian type or Imerslund-Grasbeck syndrome (I-GS). RH-MGA1 is due to selective malabsorption of vitamin B12. Defects in vitamin B12 absorption lead to impaired function of thymidine synthase. As a consequence DNA synthesis is interrupted. Rapidly dividing cells involved in erythropoiesis are particularly affected.

Cellular Component: Golgi apparatus; lysosomal lumen; protein complex; brush border membrane; endoplasmic reticulum; lysosome; coated pit; membrane; apical part of cell; endocytic vesicle; apical plasma membrane; cytoplasm; brush border; endosome

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; cobalamin transporter activity; hemoglobin binding; metal ion binding; receptor activity; calcium ion binding; cobalamin binding

Biological Process: steroid metabolic process; cholesterol metabolic process; receptor-mediated endocytosis; protein transport; in utero embryonic development; transport; lipoprotein transport; cobalamin transport; hemoglobin import; lipid metabolic process; response to nutrient

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