Insulin-like growth factor I isoform d; IGF

For Research Use Only. Not for use in diagnostic procedures.

E Coli

>95% by SDS-PAGE

Liquid; In Phosphate-Buffered Saline (pH 7.4) Containing 10% Glycerol.

0.5mg/ml (determined by Absorbance at 280nm) (lot specific)

Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.

Small volumes of IGF-1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

IGF-1, also known as Insulin-like growth factor I isoform d, is mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. IGF-1 is predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGF-1 belongs to the insulin gene family, which also contains insulin and relaxin. The IGF-1 is similar by structure and function to insulin, but has a much higher growth-promoting activity than insulin. Recombinant rat IGF-1 protein, was expressed in E Coli and purified by using conventional chromatography.

Hormones & Cytokines; Development; Signal Transduction; Metabolism; Cosmetics

SDS-PAGE

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

7.8kDa (71aa), confirmed by MALDI-TOF

insulin-like growth factor 1

insulin-like growth factor I

Insulin-like growth factor I

Igf-1; IGF-I??[Similar Products]

IGF1_RAT

growth factor; plays a major role in mammalian growth [RGD, Feb 2006]

The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca2+-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1 ().

Zumstein P., et al. (1987) J Biol Chem. 262(33): 11252-60. Rabinovsky ED., et al. (2004) Neurol Res. 26(2): 204-10.

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