Rabbit Anti-Human IGF-1 Polyclonal; Insulin-like growth factor 1 Antibody; Igf1 Antibody; IGF-I Antibody; Insulin like growth factor 1 (somatomedin C) Antibody; IGF1_HUMAN Antibody; Somatomedin-C Antibody; MGF Antibody; OTTHUMP00000195083 Antibody; Somatomedia C Antibody; Mechano growth factor Antibody; Insulin like growth factor 1 Antibody; Insulin like growth factor IA Antibody; IGF IB Antibody; IBP1 Antibody; IGF1 Antibody; Insulin-like growth factor I Antibody; IGF IA Antibody; Somatomedin C Antibody; IGF1A Antibody; IGFI Antibody; IGFIA Antibody; IGF I Antibody; Insulin like growth factor IB Antibody; IGF 1 Antibody; IGF I Antibody

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Detects ~25 kDa.

Peptide Affinity Purified

1 mg/ml (lot specific)

Store at -20 degree C.

Small volumes of anti-IGF-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Antibodies; Cell Signaling; Growth Factors; Insulin and Insulin-like; Cancer

Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

WB: 1:1000
ICC/IF: 1:100

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-IGF-1 Polyclonal Antibody (SPC-699). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-IGF-1 Polyclonal Antibody (SPC-699) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) IGF-1 Antibody. (D) Composite.

Western blot analysis of Mouse Liver cell lysates showing detection of ~21.8 kDa IGF-1 protein using Rabbit Anti-IGF-1 Polyclonal Antibody (SPC-699). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Liver cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-IGF-1 Polyclonal Antibody (SPC-699) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~21.8 kDa.

17,762 Da

insulin like growth factor 1

insulin-like growth factor I

Insulin-like growth factor I

IBP1; IGF-I; MGF??[Similar Products]

The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Sep 2015]

The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation (PubMed:21076856, PubMed:24132240). Ca2+-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb (). Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1 (PubMed:19578119, PubMed:22351760, PubMed:23696648, PubMed:23243309).

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