Full Product Name
Anti-ARL6 Antibody
Product Gene Name
anti-ARL6 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Species Reactivity
Human. Predicted: Mouse, Rat
Purity/Purification
Affinity purified
Concentration
100ug/100ul (lot specific)
Storage Buffer
PBS, pH 7.4 with 0.02% sodium azide.
Immunogen
Rabbit polyclonal ARL6(1) antibody was raised against a recombinate human ARL6 protein2-186aa (BC024239).
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Other Notes
Small volumes of anti-ARL6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ARL6 antibody
This gene belongs to the ARF-like (ADP ribosylation factor-like) sub-family of the ARF family of GTP-binding proteins which are involved in regulation of intracellular traffic. Mutations in this gene are associated with Bardet-Biedl syndrome (BBS). Alternative splicing results in multiple transcript variants of this gene. A vision-specific transcript encoding a different protein has been described (PMID: 20333246). [provided by RefSeq, May 2013]
Applications Tested/Suitable for anti-ARL6 antibody
ELISA (EIA), Immunohistochemistry (IHC)
Application Notes for anti-ARL6 antibody
ELISA titer: 1:20,000-1:80,000
Immunohistochemistry: 1:100-1:500
Optimal dilutions/concentrations should be determined by the end user
Testing Data of anti-ARL6 antibody
NCBI/Uniprot data below describe general gene information for ARL6. It may not necessarily be applicable to this product.
NCBI Accession #
BC024239
[Other Products]
UniProt Secondary Accession #
A8KA93; D3DN31[Other Products]
UniProt Related Accession #
Q9H0F7[Other Products]
Molecular Weight
21,960 Da
NCBI Official Full Name
Homo sapiens ADP-ribosylation factor-like 6, mRNA
NCBI Official Synonym Full Names
ADP-ribosylation factor-like 6
NCBI Official Symbol
ARL6??[Similar Products]
NCBI Official Synonym Symbols
BBS3; RP55
??[Similar Products]
NCBI Protein Information
ADP-ribosylation factor-like protein 6; Bardet-Biedl syndrome 3 protein
UniProt Protein Name
ADP-ribosylation factor-like protein 6
UniProt Synonym Protein Names
Bardet-Biedl syndrome 3 protein
UniProt Gene Name
ARL6??[Similar Products]
UniProt Entry Name
ARL6_HUMAN
NCBI Summary for ARL6
The protein encoded by this gene belongs to the ARF-like (ADP ribosylation factor-like) sub-family of the ARF family of GTP-binding proteins which are involved in regulation of intracellular traffic. Mutations in this gene are associated with Bardet-Biedl syndrome (BBS). Alternative splicing results in multiple transcript variants of this gene. A vision-specific transcript encoding a different protein has been described (PMID: 20333246). [provided by RefSeq, May 2013]
UniProt Comments for ARL6
ARL6: Involved in membrane protein trafficking at the base of the ciliary organelle. Mediates recruitment onto plasma membrane of the BBSome complex which would constitute a coat complex required for sorting of specific membrane proteins to the primary cilia. May regulate cilia assembly and disassembly and subsequent ciliary signaling events such as the Wnt signaling cascade. Isoform 2 may be required for proper retinal function and organization. Defects in ARL6 are a cause of Bardet-Biedl syndrome type 3 (BBS3). Bardet-Biedl syndrome (BBS) is a genetically heterogeneous disorder characterized by usually severe pigmentary retinopathy, early onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation. Secondary features include diabetes mellitus, hypertension and congenital heart disease. Defects in ARL6 are the cause of retinitis pigmentosa type 55 (RP55). RP55 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. Belongs to the small GTPase superfamily. Arf family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: G protein, monomeric, ARF; G protein; G protein, monomeric
Chromosomal Location of Human Ortholog: 3q11.2
Cellular Component: membrane coat; membrane; cytoplasm; plasma membrane; axoneme; cytosol; cilium
Molecular Function: protein binding; GTP binding; metal ion binding; phospholipid binding
Biological Process: fat cell differentiation; protein polymerization; Wnt receptor signaling pathway; visual perception; small GTPase mediated signal transduction; organelle organization and biogenesis; cilium biogenesis; brain development; intermembrane transport; protein targeting to membrane; determination of left/right symmetry; melanosome transport; regulation of smoothened signaling pathway
Disease: Retinitis Pigmentosa 55; Bardet-biedl Syndrome 3; Bardet-biedl Syndrome 1; Retinitis Pigmentosa
Product References and Citations for anti-ARL6 antibody
(1) Pretorius,P.R., Aldahmesh,M.A., Alkuraya,F.S.et al. Functional analysis of BBS3 A89V that results in non-syndromic retinal degeneration. Hum. Mol. Genet. 20 (8), 1625-1632 (2011)
Research Articles on ARL6
1. The BBSome is a coat-like ciliary trafficking complex composed of proteins mutated in Bardet-Biedl syndrome. ARL6 E108A mutation prevents BBSome recruitment to cilia.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.