Full Product Name
TLL1 siRNA (Human)
Product Synonym Names
TLL; Tolloid-like protein 1
Product Gene Name
TLL1 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O43897
Specificity
TLL1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TLL1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of TLL1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
TLL1 sirna
siRNA to inhibit TLL1 expression using RNA interference
Applications Tested/Suitable for TLL1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for TLL1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001191689.1
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NCBI GenBank Nucleotide #
NM_001204760.1
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UniProt Primary Accession #
O43897
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UniProt Secondary Accession #
Q96AN3; Q9NQS4; B2RMU2[Other Products]
UniProt Related Accession #
O43897[Other Products]
Molecular Weight
44,186 Da
NCBI Official Full Name
tolloid-like protein 1 isoform 2
NCBI Official Synonym Full Names
tolloid-like 1
NCBI Official Symbol
TLL1??[Similar Products]
NCBI Official Synonym Symbols
TLL; ASD6
??[Similar Products]
NCBI Protein Information
tolloid-like protein 1
UniProt Protein Name
Tolloid-like protein 1
Protein Family
Tolloid-like protein
UniProt Gene Name
TLL1??[Similar Products]
UniProt Synonym Gene Names
TLL??[Similar Products]
UniProt Entry Name
TLL1_HUMAN
NCBI Summary for TLL1
This gene encodes an astacin-like, zinc-dependent, metalloprotease that belongs to the peptidase M12A family. This protease processes procollagen C-propeptides, such as chordin, pro-biglycan and pro-lysyl oxidase. Studies in mice suggest that this gene plays multiple roles in the development of mammalian heart, and is essential for the formation of the interventricular septum. Allelic variants of this gene are associated with atrial septal defect type 6. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]
UniProt Comments for TLL1
TLL1: Protease which processes procollagen C-propeptides, such as chordin, pro-biglycan and pro-lysyl oxidase. Required for the embryonic development. Predominant protease, which in the development, influences dorsal-ventral patterning and skeletogenesis. Defects in TLL1 are the cause of atrial septal defect type 6 (ASD6). A congenital heart malformation characterized by incomplete closure of the wall between the atria resulting in blood flow from the left to the right atria. Belongs to the peptidase M12A family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.4.24.-; Secreted, signal peptide; Secreted; Protease
Chromosomal Location of Human Ortholog: 4q32-q33
Cellular Component: extracellular region
Molecular Function: collagen binding; zinc ion binding; metalloendopeptidase activity; calcium ion binding
Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; proteolysis; cell differentiation; skeletal development
Disease: Atrial Septal Defect 6
Research Articles on TLL1
1. This study identified TLL1 as a new susceptibility gene for PTSD.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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