Anti -NCX1 (Sodium Calcium Exchanger)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 2; NC_000002.11 (40339286..40739575, complement). Location: 2p23-p22

Polyclonal

IgG

Rabbit

Recognizes rat NCX1 (Sodium Calcium Exchanger). No significant sequence homology is detected with other NCX. Species sequence homology: mouse-100%, feline-77%, human, rabbit, monkey, bovine-72%, chicken-66%.

Affinity Purified
Purified over the peptide-Sepharose column

Supplied as a lyophillized powder in PBS, pH 7.4, 0.1% BSA. Reconstitute with 100ul sterile PBS.

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-SLC8A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Ca2+ plays a critical role in intracellular signaling. Intracellular Ca2+ levels are tightly controlled by continuos removal of Ca2+ via ATP-driven Ca2+ pump in the endoplasmic reticulum and plasma membrane, and Ca2+ transport system, the Na+/Ca2+ exchangers (NCX), in the plasma membrane. NCX can move Ca2+ either into or out of cells, depending on the net Na+, Ca2+, and K+ gradient across the membrane. In most cells, 3 Na+ are exchanged for 1 Ca2+. In mammals, at least 5 distinct genes code for the exchangers: Three NCX (NCX1, NCX2, and NCX3), and two in the NCKX family (NCKX1 and NCKX2). NCX share significant sequence homology (~70%), display 11 TM domains, a large central, intracellular hydrophilic regulatory loop between TM5 and 6, extracellular N-terminus and cytoplasmic C-terminus. The N-terminal signal peptide is cleaved off from the mature exchanger protein.

Antibodies; Abs to Ion Channel

ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)

Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Dilution: Western Blot: 1-10ug/ml using ECL.
ELISA: 0.5-1ug/ml. Control peptide N0560-20B can be used to coat ELISA plates at 1ug/ml.
Immunohistochemistry (PFFE): 5-20ug/ml. 4% paraformaldehyde.

9,406 Da[Similar Products]

solute carrier family 8 (sodium/calcium exchanger), member 1

sodium/calcium exchanger 1; Na+/Ca++ exchanger; Na+/Ca2+ exchanger; OTTHUMP00000201546; OTTHUMP00000201569; OTTHUMP00000201570; OTTHUMP00000201587; OTTHUMP00000201589; Na(+)/Ca(2+)-exchange protein 1

Ncx1 protein

Q6LAJ8_HUMAN

In cardiac myocytes, Ca(2+) concentrations alternate between high levels during contraction and low levels during relaxation. The increase in Ca(2+) concentration during contraction is primarily due to release of Ca(2+) from intracellular stores. However, some Ca(2+) also enters the cell through the sarcolemma (plasma membrane). During relaxation, Ca(2+) is sequestered within the intracellular stores. To prevent overloading of intracellular stores, the Ca(2+) that entered across the sarcolemma must be extruded from the cell. The Na(+)-Ca(2+) exchanger is the primary mechanism by which the Ca(2+) is extruded from the cell during relaxation. In the heart, the exchanger may play a key role in digitalis action. The exchanger is the dominant mechanism in returning the cardiac myocyte to its resting state following excitation.[supplied by OMIM]

NCX1: Rapidly transports Ca(2+) during excitation-contraction coupling. Ca(2+) is extruded from the cell during relaxation so as to prevent overloading of intracellular stores. Belongs to the sodium/potassium/calcium exchanger family. SLC8 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter; Membrane protein, multi-pass; Membrane protein, integral; Motility/polarity/chemotaxis; Transporter, SLC family

Chromosomal Location of Human Ortholog: 2p22.1

Cellular Component: integral to plasma membrane; basolateral plasma membrane; T-tubule; plasma membrane; Z disc; sarcolemma

Molecular Function: calmodulin binding; metal ion binding; cytoskeletal protein binding; ankyrin binding; calcium:sodium antiporter activity

Biological Process: heart morphogenesis; embryonic placenta development; cellular sodium ion homeostasis; regulation of heart rate; vascular smooth muscle contraction; post-embryonic development; calcium ion homeostasis; cardiac muscle cell development; muscle contraction; reduction of cytosolic calcium ion concentration; embryonic heart tube development; ion transport; blood coagulation; muscle fiber development; transmembrane transport; regulation of the force of heart contraction; cardiac muscle contraction

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