Anti -NCX1 (Na/Ca Exchanger)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 2; NC_000002.11 (40339286..40739575, complement). Location: 2p23-p22

Monoclonal

IgG2b

2Q186

Mouse

Recognizes mouse NCX1 (Na/Ca Exchanger). Species Crosseactivity: Mouse brain, heart, kidney, lung, liver, spleen and skeletal muscle tissues. Also reactive with rabbit and mouse cell lines.

Affinity Purified
Purified by affinity chromatography.

Supplied as a lyophilized powder. No preservatives added. Reconstitute with 50ul of sterle PBS or 40-50% glycerol for long term storage.

Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-SLC8A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Antibodies; Abs to Ion Channel

ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation and ELISA.
Dilution: Western Blot: 1:100-1:200 (ECL). Strong and specific immunolabeling of 160 and 120kD Na/Ca Exchanger (NCX1) proteins in Western Blot. In some preparations, a 70kD band breakdown fragment can be observed. Recommend proteinase inhibitors in membrane preparations.
Immunohistochemistry: 1:200-1:1000
Immunocytochemistry: 1:200-1:1000
Optimal dilutions to be determined by end user.

9,406 Da[Similar Products]

solute carrier family 8 (sodium/calcium exchanger), member 1

sodium/calcium exchanger 1; Na+/Ca++ exchanger; Na+/Ca2+ exchanger; OTTHUMP00000201546; OTTHUMP00000201569; OTTHUMP00000201570; OTTHUMP00000201587; OTTHUMP00000201589; Na(+)/Ca(2+)-exchange protein 1

Ncx1 protein

Q6LAJ8_HUMAN

In cardiac myocytes, Ca(2+) concentrations alternate between high levels during contraction and low levels during relaxation. The increase in Ca(2+) concentration during contraction is primarily due to release of Ca(2+) from intracellular stores. However, some Ca(2+) also enters the cell through the sarcolemma (plasma membrane). During relaxation, Ca(2+) is sequestered within the intracellular stores. To prevent overloading of intracellular stores, the Ca(2+) that entered across the sarcolemma must be extruded from the cell. The Na(+)-Ca(2+) exchanger is the primary mechanism by which the Ca(2+) is extruded from the cell during relaxation. In the heart, the exchanger may play a key role in digitalis action. The exchanger is the dominant mechanism in returning the cardiac myocyte to its resting state following excitation.[supplied by OMIM]

NCX1: Rapidly transports Ca(2+) during excitation-contraction coupling. Ca(2+) is extruded from the cell during relaxation so as to prevent overloading of intracellular stores. Belongs to the sodium/potassium/calcium exchanger family. SLC8 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Transporter, SLC family; Membrane protein, multi-pass; Transporter; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2p22.1

Cellular Component: basolateral plasma membrane; integral to plasma membrane; T-tubule; plasma membrane; Z disc; sarcolemma

Molecular Function: calmodulin binding; metal ion binding; cytoskeletal protein binding; ankyrin binding; calcium:sodium antiporter activity

Biological Process: heart morphogenesis; embryonic placenta development; cellular sodium ion homeostasis; regulation of heart rate; vascular smooth muscle contraction; post-embryonic development; calcium ion homeostasis; cardiac muscle cell development; muscle contraction; reduction of cytosolic calcium ion concentration; embryonic heart tube development; ion transport; blood coagulation; muscle fiber development; transmembrane transport; regulation of the force of heart contraction; cardiac muscle contraction

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