Full Product Name
Anti - NCX1 (Sodium Calcium Exchanger)
Product Synonym Names
NCX1 (Sodium Calcium Exchanger)
Product Gene Name
anti-NCX1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 2; NC_000002.11 (40339286..40739575, complement). Location: 2p23-p22
3D Structure
ModBase 3D Structure for Q6LAJ8
Host
Host: Rabbit
Source: Rat
Specificity
The 18 AA rat control peptide 100% conserved in mouse, rat, 77% in feline, 72% in human, rabbit, monkey, bovine and 66% in chicken NCX1. No significant sequence homology is detected with other NCX. The control peptide is available to confirm specificity of antibodies
Purity/Purification
Serum
Form/Format
Serum
Supplied as a liquid, neat serum, 40% glycerol.
Immunogen
An 18 AA Peptide sequence near the N-terminus of mature rat NCX1 (1). The peptide was coupled to KLH
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-NCX1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-NCX1 antibody
Ca2+ plays a critical role in intracellular signaling. Intracellular Ca2+ levels are tightly controlled by continuos removal of Ca2+ via ATP-driven Ca2+ pump in the endoplasmic reticulum and plasma membrane, and Ca2+ transport system, the Na+/Ca2+ exchangers (NCX), in the plasma membrane. NCX can move Ca2+ either into or out of cells, depending on the net Na+, Ca2+, and K+ gradient across the membrane. In most cells, 3 Na+ are exchanged for 1 Ca2+. In mammals, at least 5 distinct genes code for the exchangers: Three NCX (NCX1, NCX2, and NCX3), and two in the NCKX family (NCKX1 and NCKX2). NCX share significant sequence homology (~70%), display 11 TM domains, a large central, intracellular hydrophilic regulatory loop between TM5 and 6, extracellular N-terminus and cytoplasmic C-terminus. The N-terminal signal peptide is cleaved off from the mature exchanger protein.
Product Categories/Family for anti-NCX1 antibody
Abs to Ion Channel
Applications Tested/Suitable for anti-NCX1 antibody
ELISA, Western Blot
Application Notes for anti-NCX1 antibody
Suitable for use in ELISA, Western Blotting. Other applications not tested.
Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using ECL.
ELISA:1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity purified. Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
NCBI/Uniprot data below describe general gene information for NCX1. It may not necessarily be applicable to this product.
UniProt Primary Accession #
Q6LAJ8
[Other Products]
UniProt Related Accession #
P32418; Q14913; Q4QQG6; Q4QQH3; Q59GN4; Q6LAJ8; Q6LAJ9; Q7Z686[Other Products]
Molecular Weight
9,406 Da
NCBI Official Full Name
ncx1
NCBI Official Synonym Full Names
solute carrier family 8 (sodium/calcium exchanger), member 1
NCBI Official Symbol
SLC8A1??[Similar Products]
NCBI Official Synonym Symbols
NCX1; FLJ37694; FLJ43417; MGC119581; DKFZp779F0871
??[Similar Products]
NCBI Protein Information
sodium/calcium exchanger 1; Na+/Ca++ exchanger; Na+/Ca2+ exchanger; OTTHUMP00000201546; OTTHUMP00000201569; OTTHUMP00000201570; OTTHUMP00000201587; OTTHUMP00000201589; Na(+)/Ca(2+)-exchange protein 1
UniProt Protein Name
Ncx1 protein
UniProt Gene Name
ncx1??[Similar Products]
UniProt Entry Name
Q6LAJ8_HUMAN
Product References and Citations for anti-NCX1 antibody
??1. Low, W., et al. (1993) FEBS Lett. 316, 63.
??2. Furman, I., et al. (1993) FEBS Lett. 319, 105.
??3. Lee, S.L., et al. (1994) J Biol. Chem. 269, 14849.
??4. Nicoll, D.A., et al. (1996) J Biol. Chem. 271, 24914.
??5. Kumoro, I., et al. (1992) PNAS 89, 4769.
??6. Blaustein, M.P. and Lederer, J. (1999) Physiol Rev. 79, 763-854 (review).
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
