For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG1

MIH11

Mouse

FITC
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

IgG concentration 0.1mg/ml (lot specific)

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Small volumes of anti-CD275 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Mouse anti Human CD275 antibody, clone MIH11 detects CD275, also known as ICOS ligand (inducible T cell co-stimulator ligand). CD275 is a member of the B7 family, and interaction with its receptor CD278 (ICOS) plays an important role in T cell co-stimulation and inflammatory immune reactions. CD275 is expressed on activated B-cells, monocytes and dendritic cells and is also induced on non-lymphoid tissue cells such as endothelial cells, epithelial cells and muscle cells. CD275 has an important physiological role in the reactivation of effector/memory T cells on the endothelium, thus controlling entry of immune cells into inflamed tissue. The CD278-CD275 pathway is thought to be critical in the immune response to transplants, allergies and autoimmune diseases. MIH11 is reported to partially block the interaction between CD275 and CD278.

Flow cytometry (FC/FACS)

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/5

Staining of human peripheral blood monocytes with Mouse anti Human CD275:FITC

Staining of human peripheral blood monocytes with Mouse anti Human CD275 (MBS216277)

Staining of human peripheral blood monocytes with Mouse anti Human CD275 (MBS217328)

Staining of human peripheral blood monocytes with Mouse anti Human CD275:Alexa Fluor 488 (MBS216277A488)

Staining of human peripheral blood monocytes with Mouse anti Human CD275: RPE (MBS217329)

Staining of human peripheral blood monocytes with Mouse anti Human CD275:Alexa Fluor 647 (MBS216277A647)

20,120 Da

inducible T-cell co-stimulator ligand

ICOS ligand; B7 homolog 2; B7 homologue 2; B7-like protein Gl50; B7-related protein 1; transmembrane protein B7-H2 ICOS ligand

ICOS ligand

B7H2; B7RP1; ICOSL; KIAA0653; B7-H2; B7RP-1??[Similar Products]

ICOSL_HUMAN

ICOSLG: Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co- stimulating memory T-cell function. Belongs to the immunoglobulin superfamily. BTN/MOG family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: plasma membrane; integral to membrane

Molecular Function: receptor binding

Biological Process: hyperosmotic response; B cell activation; T cell activation; T cell costimulation; defense response; signal transduction; positive regulation of activated T cell proliferation

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.