Product Name
POU4F3, Polyclonal Antibody
Full Product Name
Goat anti-POU4F3 Antibody
Product Synonym Names
POU4F3; POU class 4 homeobox 3; BRN3C; DFNA15; MGC138412; POU domain, class 4, transcription factor 3; BRN3C antibody; DFNA15 antibody; MGC138412 antibody; POU class 4 homeobox 3 antibody; POU domain; class 4; transcription factor 3 antibody; POU4F3 antibody
Product Gene Name
anti-POU4F3 antibody
[Similar Products]
Antibody/Peptide Pairs
POU4F3 peptide (MBS427606) is used for blocking the activity of POU4F3 antibody (MBS422471)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
EAAYREKNSK PE
Species Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-EAAYREKNSKPE, from the internal region of the protein sequence according to NP_002691.1.
Note
This antibody is expected not to cross-react to the similar POU4F1 and POU4F2.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-POU4F3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-POU4F3 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-POU4F3 antibody
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Approx 40kDa band observed in Human and Mouse Heart lysates (calculated MW of 37.1kDa according to Human NP_002691.1). Recommended concentration: 0.2-0.6ug/ml.
Western Blot (WB) of anti-POU4F3 antibody
(0.2ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
NCBI/Uniprot data below describe general gene information for POU4F3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_002691.1
[Other Products]
NCBI GenBank Nucleotide #
NM_002700.2
[Other Products]
UniProt Secondary Accession #
O60557; Q2M3F8[Other Products]
UniProt Related Accession #
Q15319[Other Products]
Molecular Weight
37,052 Da
NCBI Official Full Name
POU domain, class 4, transcription factor 3
NCBI Official Synonym Full Names
POU class 4 homeobox 3
NCBI Official Symbol
POU4F3??[Similar Products]
NCBI Official Synonym Symbols
BRN3C; DFNA15
??[Similar Products]
NCBI Protein Information
POU domain, class 4, transcription factor 3
UniProt Protein Name
POU domain, class 4, transcription factor 3
UniProt Synonym Protein Names
Brain-specific homeobox/POU domain protein 3C; Brain-3C; Brn-3C
Protein Family
POU domain, class 4, transcription factor
UniProt Gene Name
POU4F3??[Similar Products]
UniProt Synonym Gene Names
BRN3C; Brain-3C; Brn-3C??[Similar Products]
UniProt Entry Name
PO4F3_HUMAN
NCBI Summary for POU4F3
This gene encodes a member of the POU-domain family of transcription factors. POU-domain proteins have been observed to play important roles in control of cell identity in several systems. This protein is found in the retina and may play a role in determining or maintaining the identities of a small subset of visual system neurons. Defects in this gene are the cause of non-syndromic sensorineural deafness autosomal dominant type 15. [provided by RefSeq, Mar 2009]
UniProt Comments for POU4F3
POU4F3: May play a role in determining or maintaining the identities of a small subset of visual system neurons. Defects in POU4F3 are the cause of deafness autosomal dominant type 15 (DFNA15). DFNA15 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Belongs to the POU transcription factor family. Class- 4 subfamily.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 5q32
Cellular Component: nucleoplasm
Molecular Function: transcription factor activity
Biological Process: auditory receptor cell differentiation; axon extension; inner ear morphogenesis; neuromuscular process controlling balance; neuron apoptosis; positive regulation of transcription from RNA polymerase II promoter; retinal ganglion cell axon guidance; sensory perception of sound; transcription from RNA polymerase II promoter; vestibulocochlear nerve development; visual perception
Disease: Deafness, Autosomal Dominant 15
Research Articles on POU4F3
1. The pou4f3 gene is regulated by ATOH1 and other transcription factors in cochlear hair cells.
Precautions
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Disclaimer
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