Product Name
POU4F3, siRNA
Full Product Name
POU4F3 siRNA (Human)
Product Synonym Names
BRN3C; POU domain. class 4. transcription factor 3; Brain-specific homeobox/POU domain protein 3C; Brain-3C; Brn-3C
Product Gene Name
POU4F3 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q15319
Specificity
POU4F3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human POU4F3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of POU4F3 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
POU4F3 sirna
siRNA to inhibit POU4F3 expression using RNA interference
Applications Tested/Suitable for POU4F3 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for POU4F3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_002691.1
[Other Products]
NCBI GenBank Nucleotide #
NM_002700.2
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UniProt Primary Accession #
Q15319
[Other Products]
UniProt Secondary Accession #
O60557; Q2M3F8[Other Products]
UniProt Related Accession #
Q15319[Other Products]
Molecular Weight
37,052 Da
NCBI Official Full Name
POU domain, class 4, transcription factor 3
NCBI Official Synonym Full Names
POU class 4 homeobox 3
NCBI Official Symbol
POU4F3??[Similar Products]
NCBI Official Synonym Symbols
BRN3C; DFNA15
??[Similar Products]
NCBI Protein Information
POU domain, class 4, transcription factor 3
UniProt Protein Name
POU domain, class 4, transcription factor 3
UniProt Synonym Protein Names
Brain-specific homeobox/POU domain protein 3C; Brain-3C; Brn-3C
Protein Family
POU domain, class 4, transcription factor
UniProt Gene Name
POU4F3??[Similar Products]
UniProt Synonym Gene Names
BRN3C; Brain-3C; Brn-3C??[Similar Products]
UniProt Entry Name
PO4F3_HUMAN
NCBI Summary for POU4F3
This gene encodes a member of the POU-domain family of transcription factors. POU-domain proteins have been observed to play important roles in control of cell identity in several systems. This protein is found in the retina and may play a role in determining or maintaining the identities of a small subset of visual system neurons. Defects in this gene are the cause of non-syndromic sensorineural deafness autosomal dominant type 15. [provided by RefSeq, Mar 2009]
UniProt Comments for POU4F3
POU4F3: May play a role in determining or maintaining the identities of a small subset of visual system neurons. Defects in POU4F3 are the cause of deafness autosomal dominant type 15 (DFNA15). DFNA15 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Belongs to the POU transcription factor family. Class- 4 subfamily.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 5q32
Cellular Component: nucleoplasm
Molecular Function: transcription factor activity
Biological Process: vestibulocochlear nerve development; neuron apoptosis; inner ear morphogenesis; sensory perception of sound; visual perception; axon extension; positive regulation of transcription from RNA polymerase II promoter; auditory receptor cell differentiation; neuromuscular process controlling balance; retinal ganglion cell axon guidance
Disease: Deafness, Autosomal Dominant 15
Research Articles on POU4F3
1. Data indicate that POUF4F3 deletion associated with hearing impairment.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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