Full Product Name
UPK2 siRNA (Human)
Product Synonym Names
Uroplakin-2; UP2; Uroplakin II; UPII
Product Gene Name
UPK2 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O00526
Specificity
UPK2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human UPK2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of UPK2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
UPK2 sirna
siRNA to inhibit UPK2 expression using RNA interference
Applications Tested/Suitable for UPK2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for UPK2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_006751.1
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NCBI GenBank Nucleotide #
NM_006760.3
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UniProt Primary Accession #
O00526
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UniProt Secondary Accession #
O00457; Q53YV0; B0YJ92[Other Products]
UniProt Related Accession #
O00526[Other Products]
Molecular Weight
19,438 Da
NCBI Official Full Name
uroplakin-2
NCBI Official Synonym Full Names
uroplakin 2
NCBI Official Symbol
UPK2??[Similar Products]
NCBI Official Synonym Symbols
UP2; UPII
??[Similar Products]
NCBI Protein Information
uroplakin-2
UniProt Protein Name
Uroplakin-2
UniProt Synonym Protein Names
Uroplakin II; UPII
UniProt Gene Name
UPK2??[Similar Products]
UniProt Synonym Gene Names
UP2; UPII??[Similar Products]
UniProt Entry Name
UPK2_HUMAN
NCBI Summary for UPK2
This gene encodes one of the proteins of the highly conserved urothelium-specific integral membrane proteins of the asymmetric unit membrane which forms urothelium apical plaques in mammals. The asymmetric unit membrane is believed to strengthen the urothelium by preventing cell rupture during bladder distention. The encoded protein is expressed in the peripheral blood of bladder cancer patients with transitional cell carcinomas.[provided by RefSeq, Sep 2009]
UniProt Comments for UPK2
UPK2: Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in regulating the assembly of the AUM. Belongs to the uroplakin-2 family.
Protein type: Endoplasmic reticulum; Membrane protein, integral
Chromosomal Location of Human Ortholog: 11q23
Cellular Component: integral to plasma membrane; apical plasma membrane; integral to endoplasmic reticulum membrane
Biological Process: epithelial cell differentiation; multicellular organismal development
Research Articles on UPK2
1. UPII was highly specific/moderately sensitive for urothelial carcinoma. May be a potentially useful marker to identify urothelial lineage and help distinguish UC from prostate cancer or, in conjunction with GATA3, from metastatic breast cancer.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
