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Keratin 1 (KRT1), Recombinant Protein

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產(chǎn)品名稱: Keratin 1 (KRT1), Recombinant Protein
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Keratin 1 (KRT1), Recombinant Protein


Keratin 1 (KRT1), Recombinant Protein  的詳細介紹
Product Name

Keratin 1 (KRT1), Recombinant Protein

Popular Item
Full Product Name

Recombinant Keratin 1 (KRT1)

Product Gene Name

KRT1 recombinant protein

[Similar Products]
Matching Pairs
Unconjugated Antibody: Keratin 1 (KRT1) (MBS2002776)
Immunogen: Keratin 1 (KRT1) (MBS2010199)
Matching Pairs
Biotin Conjugated Antibody: Keratin 1 (KRT1) (MBS2005426)
Immunogen: Keratin 1 (KRT1) (MBS2010199)
Matching Pairs
APC-CY7 Conjugated Antibody: Keratin 1 (KRT1) (MBS2043061)
Immunogen: Keratin 1 (KRT1) (MBS2010199)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Sequence
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG SEF-EGEE SRMSGECAPN VSVSVSTSHT TISGGGSRGG GGGGYGSGGS SYGSGGGSYG SGGGGGGGRG SYGSGGSSYG SGGGSYGSGG GGGGHGSYGS GSSSGGYRGG SGGGGGGSSG GRGSGGGSSG GSIGGRGSSS GGVKSSGGSS SVKFVSTTYS GVTR
Chromosome Location
Chromosome: 12; NC_000012.11 (53068520..53074191, complement). Location: 12q13.13
OMIM
113800
3D Structure
ModBase 3D Structure for P04264
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Organism
Homo sapiens (Human)
Expression System
Prokaryotic expression
Residues
Glu487~Arg644 (Accession # P04264) with N-terminal His-Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.
Other Notes
Small volumes of KRT1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for
KRT1 recombinant protein
About the Marker: Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
Applications Tested/Suitable for KRT1 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

Sequence Information of KRT1 recombinant protein
KRT1 recombinant protein Sequence Information image
SDS-Page of KRT1 recombinant protein
KRT1 recombinant protein SDS-Page image
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NCBI/Uniprot data below describe general gene information for KRT1. It may not necessarily be applicable to this product.
NCBI GI #
119395750
NCBI GeneID
3848
NCBI Accession #
NP_006112.3 [Other Products]
NCBI GenBank Nucleotide #
NM_006121.3 [Other Products]
UniProt Primary Accession #
P04264 [Other Products]
UniProt Secondary Accession #
Q14720; Q6GSJ0; Q9H298; B2RA01[Other Products]
UniProt Related Accession #
P04264[Other Products]
Molecular Weight
15.1kDa
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NCBI Official Full Name
keratin, type II cytoskeletal 1
NCBI Official Synonym Full Names
keratin 1
NCBI Official Symbol
KRT1??[Similar Products]
NCBI Official Synonym Symbols
K1; CK1; EHK; EHK1; EPPK; KRT1A; NEPPK
??[Similar Products]
NCBI Protein Information
keratin, type II cytoskeletal 1; CK-1; cytokeratin 1; cytokeratin-1; 67 kDa cytokeratin; hair alpha protein; type-II keratin Kb1; epidermolytic hyperkeratosis 1
UniProt Protein Name
Keratin, type II cytoskeletal 1
UniProt Synonym Protein Names
67 kDa cytokeratin; Cytokeratin-1; CK-1; Hair alpha protein; Keratin-1; K1; Type-II keratin Kb1
Protein Family
Keratin
UniProt Gene Name
KRT1??[Similar Products]
UniProt Synonym Gene Names
KRTA; CK-1; K1??[Similar Products]
UniProt Entry Name
K2C1_HUMAN
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NCBI Summary for KRT1
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]
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UniProt Comments for KRT1
K1: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K10.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: extracellular matrix; extracellular space; cytoskeleton; membrane; keratin filament; plasma membrane; nucleus

Molecular Function: protein binding; receptor activity; carbohydrate binding; structural molecule activity

Biological Process: retinal homeostasis; fibrinolysis; negative regulation of inflammatory response; response to oxidative stress; complement activation, lectin pathway; regulation of angiogenesis

Disease: Palmoplantar Keratoderma, Epidermolytic; Keratosis Palmoplantaris Striata Iii; Ichthyosis Hystrix, Curth-macklin Type; Palmoplantar Keratoderma, Nonepidermolytic; Epidermolytic Hyperkeratosis; Ichthyosis, Cyclic, With Epidermolytic Hyperkeratosis
Research Articles on KRT1
1. Case Report/Letter: specific mutation in 2B domain of KRT1 gives rise to mild phenotype of epidermolytic hyperkeratosis mimicking ichthyosis bullosa of Siemens.
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Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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