Product Name
Keratin 1 (KRT1), Recombinant Protein
Full Product Name
Recombinant Keratin 1 (KRT1)
Product Gene Name
KRT1 recombinant protein
[Similar Products]
Matching Pairs
Biotin Conjugated
Antibody: Keratin 1 (KRT1) (MBS2003490)
Immunogen: Keratin 1 (KRT1) (MBS2010882)
Matching Pairs
Unconjugated Antibody: Keratin 1 (KRT1) (MBS2006241)
Immunogen: Keratin 1 (KRT1) (MBS2010882)
Matching Pairs
APC-CY7 Conjugated Antibody: Keratin 1 (KRT1) (MBS2043067)
Immunogen: Keratin 1 (KRT1) (MBS2010882)
Matching Pairs
PE Conjugated Antibody: Keratin 1 (KRT1) (MBS2043068)
Immunogen: Keratin 1 (KRT1) (MBS2010882)
Matching Pairs
APC Conjugated Antibody: Keratin 1 (KRT1) (MBS2043069)
Immunogen: Keratin 1 (KRT1) (MBS2010882)
Matching Pairs
Cy3 Conjugated Antibody: Keratin 1 (KRT1) (MBS2043070)
Immunogen: Keratin 1 (KRT1) (MBS2010882)
Matching Pairs
FITC Conjugated Antibody: Keratin 1 (KRT1) (MBS2043071)
Immunogen: Keratin 1 (KRT1) (MBS2010882)
Matching Pairs
HRP Conjugated Antibody: Keratin 1 (KRT1) (MBS2043072)
Immunogen: Keratin 1 (KRT1) (MBS2010882)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG SEF-RE QIKSLNDKFA SFIDKVRFLE QQNQVLQTKW ELLQQVDTTT RTQNLDPFFE NYISILRRKV DSLKSDQSRM DSELKNMQDL VEEYRTKYED EINKRTNAEN EFVTIKKDVD SAYMTKVELQ AKADALQQDI DFFSALYQME MSQMQTQISE TNVVLSMDNN RSLDLDGIIS EVKAQYDSIC QRSKAEAETF YQSKYEELQI TAGKHGDSVR NTKMEISELN RMIQRLRSEI DGCKKQISQI QQNINDAEQR GEKALKDAQN KLNEIEDALS QCKEDLARLL RDFQELMNTK LALDMEIATY KKLLEGE
Chromosome Location
Chromosome: 15; NC_000081.6 (101845426..101850786, complement). Location: 15 F3; 15 57.06 cM
3D Structure
ModBase 3D Structure for P04104
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Organism
Mus musculus (Mouse)
Expression System
Prokaryotic expression
Residues
Arg189~Glu497 (Accession # P04104) with N-terminal His-Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.
Other Notes
Small volumes of KRT1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
KRT1 recombinant protein
About the Marker: Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
Applications Tested/Suitable for KRT1 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
SDS-Page of KRT1 recombinant protein
NCBI/Uniprot data below describe general gene information for KRT1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_032499.2
[Other Products]
NCBI GenBank Nucleotide #
NM_008473.2
[Other Products]
UniProt Primary Accession #
P04104
[Other Products]
UniProt Secondary Accession #
Q149E0; Q9D2K8[Other Products]
UniProt Related Accession #
P04104[Other Products]
NCBI Official Full Name
keratin, type II cytoskeletal 1
NCBI Official Synonym Full Names
keratin 1
NCBI Official Symbol
Krt1??[Similar Products]
NCBI Official Synonym Symbols
Krt86; Krt2-1; Krt-2.1
??[Similar Products]
NCBI Protein Information
keratin, type II cytoskeletal 1; K1; CK-1; keratin-1; keratin 86; cytokeratin-1; 67 kDa cytokeratin; type-II keratin Kb1; keratin complex 2, basic, gene 1
UniProt Protein Name
Keratin, type II cytoskeletal 1
UniProt Synonym Protein Names
67 kDa cytokeratin; Cytokeratin-1; CK-1; Keratin-1; K1; Type-II keratin Kb1
UniProt Gene Name
Krt1??[Similar Products]
UniProt Synonym Gene Names
Krt2-1; CK-1; K1??[Similar Products]
UniProt Entry Name
K2C1_MOUSE
UniProt Comments for KRT1
K1: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K10.
Protein type: Cytoskeletal
Cellular Component: extracellular matrix; extracellular space; membrane; keratin filament; plasma membrane; intermediate filament; nucleus
Molecular Function: carbohydrate binding; structural molecule activity
Biological Process: negative regulation of inflammatory response; complement activation, lectin pathway
Research Articles on KRT1
1. Absence of Krt1 caused a prenatal increase in interleukin-18 (IL-18) and the S100A8 and S100A9 proteins, accompanied by a barrier defect and perinatal lethality.
Precautions
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Disclaimer
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