Human Keratin; type II cytoskeletal 1 (KRT1) ELISA kit; CK1; EHK; EHK1; EPPK; K1; KRT1A; NEPPK; cytokeratin 1; epidermolytic hyperkeratosis 1; hair alpha protein; keratin; type II cytoskeletal 1; keratin 1

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Human KRT1. No significant cross-reactivity or interference between Human KRT1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of KRT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS909540 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the keratin 1 (KRT1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing KRT1. The ELISA analytical biochemical technique of the MBS909540 kit is based on KRT1 antibody-KRT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KRT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KRT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KRT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KRT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KRT1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KRT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

66,039 Da

keratin 1

keratin, type II cytoskeletal 1; CK-1; cytokeratin 1; cytokeratin-1; 67 kDa cytokeratin; hair alpha protein; type-II keratin Kb1; epidermolytic hyperkeratosis 1

Keratin, type II cytoskeletal 1

KRTA; CK-1; K1??[Similar Products]

K2C1_HUMAN

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]

K1: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K10.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: extracellular matrix; extracellular space; cytoskeleton; membrane; keratin filament; plasma membrane; nucleus

Molecular Function: protein binding; receptor activity; structural molecule activity; carbohydrate binding

Biological Process: retinal homeostasis; fibrinolysis; negative regulation of inflammatory response; response to oxidative stress; complement activation, lectin pathway; regulation of angiogenesis

Disease: Palmoplantar Keratoderma, Epidermolytic; Keratosis Palmoplantaris Striata Iii; Ichthyosis Hystrix, Curth-macklin Type; Palmoplantar Keratoderma, Nonepidermolytic; Epidermolytic Hyperkeratosis; Ichthyosis, Cyclic, With Epidermolytic Hyperkeratosis

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